January 2014
Restriction enzyme mediated integration and FIV lentiviral transgenesis applied to amphibians
Restriction enzyme mediated integration (REMI) transgenesis and lentiviral transgenesis are effective methods of introducing transgenes into the genome of frogs. One aquatic amphibian species, Xenopus laevis, and one land dwelling species, Litoria caerulea, were chosen as subjects for transgenesis. REMI produced X. laevis that expressed the fluorescent protein DsRed. REMI was unsuccessful in producing transgenic...
January 2014
Inhibition of Salmonella Typhimurium by bovicin HC5 associated with chelating agents and surfactants
The antimicrobial effect of bovicin HC5, a bacteriocin produced by Streptococcus bovis HC5, associated with chelating agents or surfactants was investigated against Salmonella Typhimurium. The microorganism grown in brain and heart infusion broth (BHI) was treated with bovicin HC5 at concentrations of 50, 100, 150 and up to 200 AU.mL-1; associated with chelating agents, or surfactants and growth was assessed by...
January 2014
Early plant growth promotion of maize by various sulfur oxidizing bacteria that uses different thiosulfate oxidation pathway
The objective of the work was to evaluate the plant growth promotion of maize by thiosulfate-oxidizing bacteria possessing the tetrathionate intermediate (S4I) and/or paracoccus sulfur oxidation (PSO) pathway for thiosulfate oxidation. Halothiobacillus sp. possessing the S4I and PSO pathways for thiosulfate oxidation recorded the highest phosphate solubilization (480 µg ml-1), followed by other S4I pathway bearing...
January 2014
Synergistic effect of ampicillin and gentamicin on beta-lactamase producing and methicillin resistant staphylococci
The antibacterial effect of ampicillin, gentamicin, vancomycin, methicillin and other drugs was tested on both beta-lactamase producing and methicillin resistant staphylococci. A total of 60 clinical isolates were collected from Irrua Specialist Teaching Hospital, Irrua. The results reveal that 32 (53.3%) of the staphylococci isolated produced beta-lactamase while 28 (47.7%) did not produce beta-lactamase. The...
January 2014
Characterization and immobilization of partially purified alkaline protease extracted from rhizospheric soil bacterium, Bacillus megaterium strain EN-2 and Bacillus subtilis strain EN-3
In this study, extracellular alkaline protease producing bacterial isolates EN-2 and EN-3 from the agricultural soil of C.R.C. Pantnagar were identified as Bacillus megaterium strains EN-2 and Bacillus subtilis strain EN-3 on the basis of 16S rDNA gene sequencing. During kinetic characterization, optimum pH for EN-2 and EN-3 protease activity was 10 and 9, respectively. While optimum temperature, for maximum protease...
January 2014
Isolation of two thermophilic actinobacterial strains mud volcano of the Baratang Island, India
Studies on actinobacteria of marine habitats are gaining international importance due to proven abilities of novel compound production. Information on actinobacteria of the marine environments is less and hence the present studies were optimum at higher temperature, which indicates that they are thermophilic in nature. It can be conducted that the two strains (TA-1 and TA-2) belong to two different genera viz....
January 2014
Sodium-potassium ionic ratio correlates with yeast induction from Mucor circinelloides Tieghem
A critical feature of fungal dimorphism is the morphogenetic conversion from mold to the yeast form. In the conversion of a fungus to the yeast form, little is known about the morphological changes that occur prior to the latter. A previous study from our laboratory showed that K+ plays important role in the generation of protoplasts from sporangiospores; other morphologies exhibited included thallo-arthric-,...
January 2014
The biodiversity of oleaginous microalgae in Northern Qinghai-Tibet Plateau
Microalgae represent an exceptionally diverse but highly specialized group of micro-organisms that adapt to various ecological habitats. Due to their unique environment (extremely low temperature and anoxia), the Tibetan Plateau is among the regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring and lawn. In this study, 52 microalgae from different environments in...
January 2014
Efficient extraction and rapid quantitative determination of nucleoside compounds from Cordyceps jiangxiensis, a new Cordyceps producing-cordycepin
Cordyceps, a well-known and precious traditional Chinese medicine, has received increasing attention worldwide due to its outstanding curative effects for different diseases. Nucleosides are the main active compounds of Cordyceps, and are usually the chemical marker used for the quality control of Cordyceps and its bioproduct. In this study, an optimal condition for extracting nucleosides in Cordyceps jiangxiensis was...
January 2014
Occurrence of Salmonella spp. in laying hens during cycles of molting
The main objective of this study was to determine the presence of Salmonella spp. in 30 laying hens of the third cycle. Samples were taken from the digestive tract, cloaca, feces, and organs. The sampling of feces and cloaca was conducted first, followed by the other tissues at necropsy. Feces were taken from the floor of the cages during five weeks. Thirty (30) samples of cloaca were taken at random from five hens. The...
January 2014
Incidence and anti-microbial resistance profile of Group B Streptococcus (GBS) infection in pregnant women in Nsukka, Enugu State, Nigeria
This study was performed to determine the incidence and antimicrobial resistance profile of Group B Streptococcus in pregnant women in Nsukka, Enugu State. From January 2010 to July 2011, vaginal swab and rectal swab samples were obtained from 200 pregnant women of gestational age from 24 to 37 weeks that were attending antenatal clinic at Bishop Shanahan Memorial Hospital and Nsukka District Hospital. Vaginal and...
January 2014
Evaluation of Lactobacillus and Bacillus-based probiotics as alternatives to antibiotics in enteric microbial challenged weaned piglets
The present study investigates the efficacies of two probiotic products as alternative to antibiotics on growth performance, nutrient digestibility, immunity, and fecal microbiota in piglets challenged with Salmonella enterica serovar Typhimurium KCTC 2515 and Escherichia coli KCTC 2571. Ninety-six 28-day-old piglets were randomly allotted to four dietary treatments consisting of four replicate pens with six piglets...
January 2014
Antibiotic resistance pattern of methicillin-resistance and coagulase-negative Staphylococcus isolates among hospitalized patients at a tertiary hospital in Gansu, North-western China
This study aimed at determining the prevalence and antibiotic resistance pattern of methicillin resistant Staphylococcus aureus (MRSA) and methicillin resistant coagulase negative staphylocci (MRCoNS) isolated from various clinical specimens obtained from hospitalized patients in a Tertiary Hospital in Gansu, North-western, China from 2008-2011. Bacterial isolates were identified morphologically and biochemically using...
January 2014
Microbiological comparison of microwave and traditional thawing processes for poultry meat
Health agencies official has been convinced of the efficiency of microwave based process mainly due to its bactericidal property and safety (non-ionizing radiation). However, many biotechnological aspects of this new method are still unknown and have raised safety concerns. This study aimed to validate the application of the thawing process of a large poultry meat block under high frequency microwave oven (2.45 Ghz...
January 2014
The antibacterial effect of phyto-mediated silver nanoparticles produced from Ocimum sanctum L. (Lamiaceae) leaf extract on textile fabrics
The Ocimum sanctum (holy thulsi) leaves extract perform as a reducing and capping agent in the formation of silver nanoparticles. A UV-VIS spectrum of the silver nanoparticles showed a peak at 421 nm. The morphology and size of the silver nanoparticles were carried out by the transmission electron microscope (TEM) and scanning electron microscope (SEM). The sizes of the synthesized silver nanoparticles were found to be...
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