September 2012
Conventional and molecular characterization of Trichophyton rubrum
Different studies illustrated that Trichophyton rubrum, among all species of Trichophyton, is the most prevalent and consequently the most important genus. T. rubrum as a worldwide filamentous pathogen fungus can infect human keratinized tissue (skin, nails and rarely hair), and causes dermatophytosis. Researchers use two general methods for the identification of dermatophytes namely,...
September 2012
Inhibitory effect of some plant extracts on clinical isolates of Staphylococcus aureus
Accumulating data have been dramatically increasing on multiple drug resistantStaphylococcus aureus isolates that are incriminated in nosocomial and community acquired infections causing high mortality and morbidity. Accordingly, 70 resistant isolates were collected from King Fahad General Hospital and National Guard Hospital, Jeddah, Saudi Arabia in an attempt to find alternative antimicrobial substances from...
September 2012
Role of CSE1034 in bacterial lipids and polysaccharides involved in biofilm formation: a comparison with other drugs
In the present investigation minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) and CSE1034 (Sulbactomax) was compared with piperacilline+tazobactam, amoxyclave, ceftriaxone, ceftriaxone+sulbactam and cefoperazone+ sulbactam in planktonic and sessile cells of Acinetobacter baumannii, Enterobacter cloacae and Pseudomonas aeruginosa. MICs were determined by broth...
September 2012
Analytical specificity and sensitivity determination of 16SrRNA gene based diagnostic polymerase chain reaction (PCR) for molecular detection of Coxiella burnetii
Coxiella burnetii, the cause of Q-fever, is a widespread zoonosis. Classical methods for its isolation are time consuming and risky, requiring biosafety level 3 laboratory. However, this limitation is omitted in molecular detection. The aim of this study was to evaluate the use of 16SrRNA gene in molecular detection of C. burnetii. For this purpose, specific primers were designed for this gene and a...
September 2012
Effect of Bacillus cereus Br on bacterial community and gossypol content during fermentation in cottonseed meal
To define new methods for the detoxification of free gossypol (FG) within cotton seed meal (CSM), FG during CSM fermentation with Bacillus. cereus Br were measured. Four conditions were studied: CSM inoculated with Br (Br + CSM), CSM without any inocula (CSM), sterilized CSM inoculated with Br (Br + sCSM), and sterilized CSM without any inocula (sCSM). Samples were taken at various times during the...
September 2012
Identification and characterization of a fungal strain with lignin and cellulose hydrolysis activities
A lignin and cellulose-degrading fungal strain Bio-1 was isolated from the soil of Yuelu Mountain in China. It was identified as a member of the genus Cladosporium by 18s rDNA, ITS sequences analysis and morphological characters. Bio-1 could hydrolyze at least 35% of the alkaline lignin in 4 days when incubated in screening liquid medium. Both laccase and cellulase activities could be detected in the...
September 2012
Survival of microorganisms in high pressure treated minced meat during chilled storage and at pH and temperature mimicking gastrointestinal tract
Minced meat samples containing 8.3 × 106 cfu/g determined by total aerobic count (TAC) were subjected to pressures between 259 to 540 MPa for 3 to 17 min at 6°C. A day after pressure treatment, no survival on Plate Count Agar (PCA) was detected in the samples that were treated at and above 300 MPa for 10 min or more after 24 h incubation. However, survivors were detected in the pressure treated samples,...
September 2012
Efficacy and toxicity of neutralizers against disinfectants and antiseptics used in vaccine production facility
Effective neutralization of chemical biocide is the first step in the accurate evaluation of antiseptics and disinfectants to avoid overestimation of the biocide activity. The aim of this study is to screen novel neutralizers (or neutralizer combinations) against different antiseptics and disinfectants that are highly used in the principle vaccine production facility in Egypt. Neutralizer efficacy (NE) ratios were...
September 2012
Effects of essential oil extracted from Citrullus colocynthis (CCT) seeds on growth of phytopathogenic bacteria
Citrullus colocynthis (CCT) is a non-hardy, herbaceous perennial vine, branched from the base. Originally from Tropical Asia and Africa, it is now widely distributed in the Sistan phytogeographic region of Iran. In a search of alternative ways to control plant disease, essential oil from seeds of CCT was examined for antibacterial properties. The seeds are edible and have a high oil content with a large...
September 2012
Development and evaluation of a novel TaqMan fluorescence probe-based real-time reverse transcriptase polymerase chain reaction assay for detection and quantification of West Nile virus
In order to improve and accelerate the detection of West Nile virus (WNV), a rapid and specific real-time reverse transcription polymerase chain reaction (rtRT-PCR) was established. Primers and probe were designed according to the conservative sequence of capsid protein gene of WNV. Tenfold successive dilutions of positive WNV DNA were used to measure the sensitivity of rtRT-PCR. The amplifying curve showed that this...
September 2012
Microbial water quality in the upper Olifants River catchment: Implications for health
The Olifants River has been described as one of the most environmentally polluted rivers in Southern Africa, particularly in the upper catchment. Untreated water from the catchment may be used for many purposes including irrigation, recreation, consumption, and domestic purposes. As poor quality water remains a leading cause of morbidity and mortality, it is essential to understand the health risks that the water...
September 2012
Partial characterization of a bacteriocin produced by Lactobacillus salivarius isolated from oral cavity of desert foxes
Bacteriocin production by animal-associated bacteria is considered as a natural defense against pathogens. So, the objectives of this work were to isolate, characterize, and screen lactic acid bacteria producing antimicrobial compounds from oral cavity of desert foxes. Forty one isolates of lactic acid bacteria were isolated and screened for their ability to produce antimicrobial compounds. Only four...
September 2012
DNA viral infections and transient bone marrow failure in southern Iran
Some viral infections may have roles in decreasing the myeloid and erythroid progenitor cells leading to hematopoietic crisis and bone marrow failure. In this research, the potential capacity of some DNA viruses were studied in patients with transient bone marrow failure. In a cross sectional study the plasma samples of 27 patients with clinically confirmed transient bone marrow failure were collected for 2 years. The...
September 2012
DNA viral infections and transient bone marrow failure in southern Iran
Some viral infections may have roles in decreasing the myeloid and erythroid progenitor cells leading to hematopoietic crisis and bone marrow failure. In this research, the potential capacity of some DNA viruses were studied in patients with transient bone marrow failure. In a cross sectional study the plasma samples of 27 patients with clinically confirmed transient bone marrow failure were collected for 2 years. The...
Advertisement
Advertisement
