African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 357

Full Length Research Paper

Reverse-flow-injection analysis (FIA) for the determination of Vitamin C in pharmaceutical formulation with chemiluminescence detection

Rebwar O. Hassan* and Azad T. Faizullah
Chemistry Department, College of Science, University of Salahaddin, Erbil-Iraq.  
Email: [email protected]

  •  Accepted: 23 May 2011
  •  Published: 10 October 2011


A reverse flow injection chemiluminescence (CL) method for the fast and simple determination of vitamin C has been developed. The CL light observed after luminal-H2O2 aqueous solution was added to the on-line formed Br2 solution from mixing of HBr and KBrO3 solutions was inhibited in the presence of Vitamin C. Based on these facts, a determination method of Vitamin c was developed. In order to obtain the optimum experimental conditions, effects of different variables on the chemiluminescence intensity and proposed flow-injection analysis (FIA) system were investigated. The calibration curve obtained under optimum conditions was linear over the range from 1.0 to 16.0 µg/ml with correlation coefficient R = 0.9959 and detection limit of 0.4 µg/ml. The proposed method has been successfully applied to the determination of Vitamin C content in pharmaceutical formulation. The obtained results are in good agreement with an iodimetric method reported in British Pharmacopeia.


Key words: Reverse-FIA, chemiluminescence, vitamin C, pharmaceutical formulation.


Abbreviations: CL, Chemiluminescence; AA, ascorbic acid; FIA, flow-injection analysis;DDW, deionized-distilled water; RSD, relative standard deviation.