African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 357

Full Length Research Paper

Chemical composition and biological activity of the volatile oils of Hyptis spicigera against Trypanosoma brucei brucei, (Tbb) found in Northern Nigeria

Z. Ladan1*, J. O. Amupitan2, O. A. Oyewale2, E. M. Okonkwo1, E. O. Ladan3, B. Odjobo1 and N. Habila1
1National Research Institute for Chemical Technology, Private Mail Bag 1052, Zaria-Nigeria. 2Department of Chemistry, Ahmadu Bello University, Zaria-Nigeria. 3 National Agricultural Research and Extension Liaison Services, Ahmadu Bello University, Zaria-Nigeria
Email: [email protected]

  •  Accepted: 23 February 2011
  •  Published: 30 April 2011


The hydrodistillation of the fresh leaves of Hyptis spicigera gave a colourless volatile oil with yield of 0.65%.The volatile oil gave forty compounds on the basis of  gas chromatography-mass spectroscopy (GC-MS) with low composition of cineole(4.11%) and caryophyllene (2.61%) while α-pinene (12.16%) β-pinene(9.47%) and α-phellandrene (10.19%) were predominant compounds. The biological activity of the volatile oils was evaluated in vitro for activity against Trypanosoma brucei brucei (Tbb) and was found to possess anti-trypanosoma activity in vitro in a dose dependent pattern at 0.5 µg/ml in 6 min. This activity showed the volatile oils from H. spicigera leaves to be a potential trypanocide.


Key words: Hyptis spicigera, gas chromatography-mass spectroscopy,Trypanosoma brucei brucei, trypanosome, volatile oils.