Abstract

High performance liquid chromatography with diode array detector was used to determine the ememactin residue in ground water in Lahore suburban area. The samples were extracted with chloroform through solvent extraction by using a separating funnel. The chemicals used were chloroform, methanol (HPLC grade) and acetonitrile (HPLC grade). Mobile phase used was acetonitrile (98%) and methanol (2%). 10 samples were analyzed and emamectin benzoate was detected in 5 samples. Emamectin benzoate residue was detected in terrestrial water and in ground water it was present in trace amount. The concentration range varies from 0.04 to 1.4 ppm of terrestrial field water. With respect to toxicity, it is class II pesticide by W.H.O. Presence of pesticides in large amounts can create a lot of diseases in human body.

Key words: New York codes rules and regulations (NYCRR), reference dose (RfD),United States environmental protection agency (USEPA).