African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 357

Full Length Research Paper

Biosynthesis of silver nanoparticles using Garcinia kola and its antimicrobial potential

Labulo Ayomide Hassan
  • Labulo Ayomide Hassan
  • Department of Chemistry, Federal University of Lafia, Lafia, Nasarawa State, Nigeria.
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Adesuji Temitope Elijah
  • Adesuji Temitope Elijah
  • Department of Chemistry, Federal University of Lafia, Lafia, Nasarawa State, Nigeria.
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Oseghale Charles Ojiefoh
  • Oseghale Charles Ojiefoh
  • Department of Chemistry, Federal University of Lafia, Lafia, Nasarawa State, Nigeria.
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Omojola Joseph
  • Omojola Joseph
  • Department of Physics, Federal University Lafia, Lafia, Nasarawa State, Nigeria.
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Bodede Olusola Sunday
  • Bodede Olusola Sunday
  • Department of Chemistry, College of Agriculture, Engineering and Science, University of Kwazulu- Natal, South Africa.
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Dare Enock Olugbenga
  • Dare Enock Olugbenga
  • Department of Chemistry, Federal University of Lafia, Lafia, Nasarawa State, Nigeria.
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Akinsiku Abimbola Anuoluwapo
  • Akinsiku Abimbola Anuoluwapo
  • Chemistry Department, Covenant University, Ota, Ogun State, Nigeria.
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  •  Received: 11 August 2015
  •  Accepted: 05 November 2015
  •  Published: 31 January 2016

Abstract

We have investigated the green synthesis and antimicrobial activity of silver nanoparticles using Garcinia kola.  Aqueous extract of G. kola was used to reduce AgNO3 solution to obtain black nanocrystal of silver nanoparticles. Antimicrobial activity of the silver nanoparticles was tested against Escherichia coli, Klebsiella pneumonia, Bacillus subtilis, Staphylococcus aureus, Aspergillus niger, Rhizopus stolonifer and Candida albican. The synthesized silver nanoparticles  was characterized using UV-vis spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. UV-visible exhibits prominently the characteristic surface plasmon resonance at around 427 nm. A remarkable particle growth onset of 10 min was observed while the reaction ended within 30 min. The XRD analysis shows that the synthesized silver nanoparticles are crystalline in nature and well-dispersed silver nanoparticles with an approximate size of 10.4 nm were observed on TEM. The synthesized silver was found to possess good antimicrobial activity against all the tested microbes. The application of the green synthesized nanoparticles can be used in many fields such as foods, beverages, cosmetics and medicine.

 

Key words: Silver, nanoparticles, green synthesis, Garcinia kola seed extract, transmission electron microscopy, antimicrobial activity.