African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 368

Full Length Research Paper

Preliminary characterization of some natural dyes

Mercy Amarachukwu Ezeokonkwo
  • Mercy Amarachukwu Ezeokonkwo
  • Department of Pure and Industrial Chemistry, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria.
  • Google Scholar
Sunday Nwankwo Okafor
  • Sunday Nwankwo Okafor
  • Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria.
  • Google Scholar
Evelyn Uloma Godwin-Nwakwasi
  • Evelyn Uloma Godwin-Nwakwasi
  • Department of Chemistry, Gregory University, Uturu, Abia State, Nigeria.
  • Google Scholar


  •  Received: 27 July 2018
  •  Accepted: 12 September 2018
  •  Published: 30 September 2018

 ABSTRACT

A preliminary study on the chemical structure of dyes from Rothmannia hispidia, Pterocarpus osun and Terminalia superba was made using chemical tests, UV-visible and infrared spectroscopies. R. hispidia dye was found to contain an alkyl amino group (-NHR) and carbon-carbon double bond conjugated with a carbonyl (C = O) group, and also showed maximum absorption at 595 nm in the visible region. P. osun dye contains conjugated systems, hydroxyl (-OH) and amino (NHR) groups and showed maximum absorption at 506 nm in the visible region whereas T. superba dye is made up of conjugated system, nitro (NO2) and hydroxyl (-OH) groups. It showed maximum absorption at 478 nm in the visible region.

Key words: Rothmannia hispidia, Pterocarpus osun, Terminalia superba, dyes, characterization.

 


 INTRODUCTION

Generally, dyes are used for colouring of foods, drugs, cosmetics, leather, petroleum products, and textiles materials among other things. These materials are dyed for different purposes. For instance, in leather industry, one of the reasons for dyeing the leather is to make it adaptable for fashion styling (Opara et al., 2014). Petroleum products are coloured for identification of fuel adulteration (Ezeokonkwo and Okoro, 2012) and for differentiation of various petroleum products (Rostad, 2010, Meghmani Dyes and Intermediates, 2011). Most substances are generally dyed to enhance appearance and aesthetic value of the finished product. In recent times, many people are becoming more conscious of the need to use natural dyes in food colouring as against synthetic dyes (Dweck, 2009). Some of the approved dyes are being delisted due to  legislative  action  as  well as consumer interest (Garcia and Cruz-Remes, 1993). Again, natural food colourants contain some biological active components such as lycopene, carotenes, canthaxanthin and quercetin, which play vital role in human health (Okafor et al., 2016). Turmeric (a yellow dye) is a good colouring agent (Vankar et al., 2007), which is used as spice and as natural food colorant. Turmeric has also been reported to have a powerful antiseptic effect that revitalizes the skin; while indigo, a dark blue dye has a cooling sensation (Grover and Patni, 2011). Several researches have reported on the anti-diabetic effects of many medicinal plants including Rothmannia hispidia and Pterocarpus osun dyes, and this has resulted in an increase in the number of people who use these natural compounds to control their disease (Pius et al., 2016; Saki  et  al.,  2014).   All  these information have led to increase in demand for natural dyes in food industry (Okafor et al., 2016). There is also a growing interest in the use of natural dyes in textile coloration, which has been attributed to the strict environmental measures imposed by many countries (Kamel et al., 2005). Synthetic dyes have been associated with toxicity and allergic reactions, whereas natural dyes are non-toxic, non-allergic, non-carcinogenic and more environmentally friendly (Semwal et al., 2012). Natural dyes have better biodegradability and generally show a higher compatibility with the environment. Again, there is a reduction in the use of synthetic dyes as against natural dyes, as histological stain, due to their hazard to human and animal health (Avwioro et al., 2005). According to Morrison (Morrison, 2015), there are four reasons why natural dyes should be chosen over synthetic dyes. First, clothings coloured with natural dyes reduces the challenge of toxic runoff that could be suffered when synthetic dyes are used in textile and dyeing process. Secondly, using dyes from plants that grow in our environment eliminates the problems associated with production of synthetic dyes. Thirdly, natural dyes are non-toxic to work with. Fourthly, in working with natural dyes one gains enlightening experience from direct connection with nature.

Dyes owe their colouring effect to their ability to exhibit a number of characteristics, which include presence of at least one chromophore and one auxochrome, conjugated system, resonance, and ability to absorb light in the visible region (Abrahart, 1977; Gürses et al., 2016). Chromophores are groups with multiple bonds responsible for the colour of a compound. Examples include nitro (-NO2), nitroso (-NO), azo (-N=N-), azoxy (-N=N-O-) and conjugated system ((-CH=CH-)n. Auxochromes on the other hand are colour enhancers. They are groups that make coloured substances act as dyes. Some examples of auxochromes are OH, Cl, COOH, SO3H, NH2, NHR, NR2 and OR.

Many plants in Nigeria are sources of natural dyes (Akpuaka, 1992; Nnabugwu and Okoro, 2012), among which are R. hispidia, P. osun and Terminalia superba. Some of the local names by which R. hispidia is known in Nigeria include: “okukim”, “obong”, “asun”, “asogbodu”, “uriohia” and “owuruokumuo” (Antai et al., 2010). P. osun also has many common names depending on the country and location. Some of them are Vene in French, Palissandre in Senegal, Kino in Gambia, Bani or Banuhi in Burkina Faso, Madubiya in Northern Nigeria and Osundudu in Southwest Nigeria (Shobayo et al., 2015). T. superba has several common names, such as yellow pine, white afara, limba, black korina (Englis); limba, frakè (French), limba (German), akom (Spanish), mwalambe (Spanish), and afara, afa (Yoruba-Western Nigeria) (Orwa et al., 2009). Crude extracts from R. hispidia, P. osun and T. superba are used locally by some women in Eastern part of Nigeria for body beautification. They are also used locally as a medication for the treatment of chicken-pox in children (Akpuaka, 1992). Literature has shown that R. hispida has been used for the treatment of many ailments such as diabetes mellitus (Antai et al., 2005) and skin infections (Antai et al., 1995). Pterocarpus species is one of such plants which have been used for treatment of type 2 diabetes (Mukherjee et al., 2006). The stem bark powder of Pterocarpus spp. has also been used in treating diarrhea and the wood powder has been externally applied in the treatment of inflammations, headache, mental aberrations, and ulcers (Krishnaveni et al., 2000). T. superba has broad spectrum against the growth of betalactamin multi-resistant bacteria (Anago, 2009). The bark of the plant is used in treatment of some broncho-pulmorary ailments (Akoegninou et al., 2006), diarrhea and gonorrhea (Neuwinger, 2000). Other useful properties exhibited by T. superba include antifungal (Ahon et al., 2011) and hypoglycemic activities (Wansi et al., 2007). In addition to the aforementioned, the dyes from R. hispidia, P. osun and T. superba plants have been shown to be potential dyes for colouring gasoline, diesel, kerosene, and candle wax (Nnabugwu and Okoro, 2012). These dyestuffs and many others (Akpuaka, 1992; Nnabugwu and Okoro, 2012; Akpuaka et al., 2001) have been extracted and isolated from many plants in Nigeria, but not much work has been done to investigate their chemical structures. Considering the several applications of these dyes and the growing interest in the use of natural dyes, there is urgent need to elucidate the structures of these dyes. Moreover, the knowledge of the dye structures is useful for forensic investigations and the study of art history. The present preliminary work investigated the basic chromophores and auxochromes present in dyestuffs from R. hispidia, P. osun and T. superba using chemical tests, UV-visible and infrared spectroscopies. This is a vital step in the right direction towards understanding the makeup of some of these natural dyes.

 


 MATERIALS AND METHODS

Melting points were determined on a Fisher John’s melting point apparatus and are uncorrected. Ultraviolet and visible spectra were obtained on a Unico-Uv2102 PC spectrophotometer using 1 cm quartz cells. The solvent used was ethanol or chloroform as the case may be. The absorption maxima were recorded in nanometers (nm). Infra red (FTIR) spectra were obtained as KBr discs on a magna-IR system 750 spectrophotometer. Chemical analysis was done at the Post-Graduate Laboratory, Department of Pure and Industrial Chemistry, University of Nigeria, Nsukka.

Extraction and isolation of dyestuffs

The plant materials were collected at Nsukka in Enugu State, Nigeria, and were identified by Mr A. O. Ozioko of Department of Botany, University of Nigeria, Nsukka. The dyestuffs were extracted and isolated as reported in the literature (Nnabugwu and Okoro, 2012).

Dyestuff from R. hispidia

About 275 g of R. hispidia seeds were crushed using a grinder and then soaked in 55 ml distilled water for 72 h in an airtight container. The soaked seeds were later agitated and there after filtered with a Buckner funnel. The light yellow filtrate was stirred vigorously with a magnetic stirrer for several hours until a dark blue solution was obtained. The solution was kept at room temperature for 2 weeks during which the dark blue dye precipitated out and collected at the bottom of the containing vessel as sediment. The supernatant liquid was poured out while the dark blue R. hispidia dye sediment was air-dried at room temperature.

Dyestuff from P. osun

About 150 g of ground stem wood of P. osun was steeped in 600 ml of ethanol (96%) for 72 h. After agitation, the steeped wood was filtered. The dark-red filtrate was concentrated via simple distillation to about one-third its original volume. 600 ml of water was added to the concentrate so as to precipitate the dye. After filtration, the precipitate was collected and purified by recrystallization from 96% ethanol and distilled water mixture (3:1) along with activated charcoal. The dark-red dye was dried at room temperature.

Dyestuff from T.superba

About 200 g of the T. superba stem wood was ground and soaked in 1.3 L of 96% ethanol for 72 h. The wood was agitated and filtered to obtain an orange-red filtrate. The filtrate was concentrated by simple distillation followed by evaporation through reduced pressure. An orange-red dye obtained was purified by recrystallization from 96% ethanol and 0.5 g charcoal.

Qualitative analysis

The dyes being organic compounds were qualitatively analyzed by standard methods used for analysis of organic compounds as described in Vogel (Furniss et al., 1989). Specifically, the following tests were carried out on the dyestuffs, including melting point determination.

Ignition test

About 0.01 g of each of the dyestuffs was placed in an ignition tube and heated until ignition occurred.

Solubility test

The solubility tests of the dyestuffs in 1 ml portion of ether, water, 5% NaOH, 5% NaHCO3 and 5% HCl were carried out using 0.1 g of the dyestuff in each case.

Sodium fusion test (Lassaigne’s test)

About 50 mg of each of the dyestuffs was placed in an ignition tube and four pieces of metallic sodium (2 mm cube) were added to each of the tubes. The tubes were heated gently at first (in a Burnsen burner) and then more vigorously until fumes have ceased to evolve. While the tubes were still red hot, each was dipped in a clean mortar containing about 10 ml of distilled water. The tubes were then crushed inside the mortar and the solution was filtered. The filtrates were used to test for the presence of sulphur, nitrogen and halogens.

 (i) Test for Sulphur: Few crystals of sodium nitroprusside were added to 5 ml of each of the filtrates. Purple or deep blue violet colour indicates the presence of sulphur.

(ii) Test for Nitrogen: A little quantity of ferric chloride was added to 5 ml of each filtrate and heated. The hot solution was cooled under tap and few drops of ferric chloride were added to it followed by acidification with dilute sulphuric acid. Blue or greenish blue colour indicates the presence of nitrogen.

(iii) Test for halogens: Concentrated HNO3 was added to 5 ml of each filtrate, heated and cooled under tap. Few drops of silver nitrate solution were then added.  Precipitate indicates the presence of a halogen.

Test for functional groups

Specific tests for functional groups were done on the dyestuffs to determine the functional groups present in them.

Test for aldehydes and ketones

(i) About 0.5 g of each dyestuff was added differently to 2, 4-dinitrophenyl hydrazine in ethanolic phosphoric acid. Dark red precipitate indicates the presence of aldehyde or ketone.

(ii) Tollen’s reagent test: 2 ml of Tollen’s reagent was added to each of the dyestuffs and then heated to 35°C. Silver mirror indicates the presence of aldehyde while absence of silver mirror indicates the presence of ketone.

Test for amines

(i) HCl sodium nitrite test: Concentrated HCl was added to each of the dyestuffs and cooled. Then sodium nitrite was added to the solution. A brown precipitate indicates the presence of aromatic amines.

(ii) Acetyl derivative: 6 ml of water and 0.2 ml of acetic anhydride were added to 0.2 g of each of the dyestuffs.  Formation of a derivative (gray colour) confirms the presence of amines.

Test for phenols

(i)  Methanol in iron (III) chloride test: Methanolic anhydrous iron (III) chloride solution was added to a solution of the dyestuff. A green solution indicates the presence of monohydric phenol.

(ii)  Neutral iron (III) chloride test: Neutral iron (III) chloride solution was added to a few crystals of the dyestuff. Brick red solution indicates the presence of monohydric phenols.

(iii) Benzoate derivatives: 10 ml of dilute sodium hydroxide and 0.6 ml of benzoyl chloride were added to 0.6 g of the dyestuff. Formation of the solid derivative indicates the presence of phenols.

Test for Nitro group

Each dyestuff was mixed with zinc dust and concentrated HCl and then heated. The cooled solution was tested for aromatic amine. Also, the presence of brown precipitate indicates the presence of nitro group.

Test for alkaloids

About 0.2 g  of each of the dyestuffs was boiled with 2 ml of 2% HCl on a steam bath. The mixture was cooled, filtered and 1 ml of a portion of each of the filtrate was treated with 2 drops of the following reagents:

(i) Dragendorff’s Reagent: Dirty-green precipitate indicates the presence of alkaloids.

(ii) Mayer’s Reagent: A blue precipitate indicates the presence of alkaloids.

(iii) Wagner’s Reagent: A reddish-brown precipitate indicates the presence of alkaloids.  

(iv) Picric acid (1%): A green precipitate indicates the presence of alkaloids.

Application of the dyestuffs on textile fibres

R. hispidia dyestuff: The dye bath, which is the vat, is made in two parts, the stock liquid and the vat (Finar, 2003). The stock solution is made up of water (10 ml), caustic soda (0.5 g) dissolved in water, dyestuff (0.2 g) and sodium hydrosulphite. To prepare the vat, 40 ml of warm water (38°C) was poured into a 250-ml beaker and about 0.2 g of sodium hydrosuphite sprinkled over the surface. The stock solution was then carefully poured into the vat solution and the mixture stirred gently. Four pieces of pre-washed fabrics (cotton, silk, polyester and nylon) were wetted in water and carefully put into the dye bath. They were left in the dye bath for about 20 min after which they were pulled out, allowed to drip out and air-dried. They were allowed to dry under direct sun light.

P. osun dyestuff:  1.0 g of the dye was dissolved in 550 ml ethanol heated to 70°C. Pieces of pre-washed white cotton, silk, polyester and nylon were immersed in the dye bath and the temperature maintained at 70°C for 30 min. The fabrics were then removed, washed with cold water and dried at room temperature.

T. superba dyestuff: About 0.4 g of the dyestuff was dissolved in 30 ml of ethanol heated to 70°C. The dye bath was used to dye white cotton, silk, polyester and nylon as in P. osun.

Colour fastness of the dyestuffs

The dyed fabrics were subjected to fastness tests to determine the fastness of the dyestuffs to washing, sunlight, acid and alkali.

(i) Fastness to washing: All the dyed fabrics were soaked in laundry soap for 1 h, washed and rinsed with cold water. The fabrics were then allowed to dry at room temperature.

(ii) Fastness to sunlight: The dyed fabrics were exposed to sunlight for 10 h every day for 2 days.

(iii) Fastness to acid (10% HCl): Pieces of dyed fabrics were spotted with dilute HCl (10%) and allowed to dry under room temperature.

(iv) Fastness to alkali (10% NH4OH): Pieces of the dyed fabrics were immersed in dilute ammonium hydroxide solution (10%) for 1 min. The fabrics were removed, and dried under room temperature.

 


 RESULTS AND DISCUSSION

Qualitative analysis

All the dyestuffs burn with smoky flame leaving no residue. This shows that they are all aromatic compounds. The inability of the R. hispidia dye to dissolve in any of the normal solubility test solvents, but very soluble in alkaline sodium hydrosulphite is consistent with the solubility of vat dyes. The solubility tests also show that P. osun and T. superba are probably phenols. However, it should be noted that the presence of more than one functional group might have such a profound effect on the solubility of the dyestuff that it is often impossible to make deductions about the functional groups present from solubility data (Jansen and Cardon, 2005). The chemical analysis further revealed that the R. hispidia dye is an alkaloid and thus contains basic nitrogen. It also contains a ketone carbonyl group (RCOR’). The presence of a tertiary amine was observed in this dye, contrary to the expected secondary amine in an indigo dye. This observation is attributed to possible tautomerism in the dye whereby one of the –NH groups rearranges to –N=C (Scheme 1).

 

 

The sodium fusion test shows that the P. osun dye contains nitrogen. However, the alkaloid test was negative indicating that the nitrogen present in the dye is not basic. The dye also contains phenolic and amino groups. The T. superba dye also contains nitrogen, which is not basic. Also, there is the presence of nitro and phenolic groups in the dye (Table 1).

 

 

Spectral analysis

The second column in Table 2 shows the UV-Visible spectra of the dyes. In R. hispidia dye, the peak within the region of 595 nm is due to ππ* transition resulting from multiple conjugation. This absorption band occurs in the yellow region and so the compound is blue (that  is,  complementary  colour).  The   band  at  324 nm could have resulted from nπ* transition of double bonded hetero-atom  (Finar,  2003), C = O, whereas the peak at 294 nm denote the absorption  band of ππ* transition resulting from conjugation which is the characteristic of benzene  derivative  (Finar,  2003).  P.   osun   dye showed absorption band at 474 to 506 nm which is due to ππ* transition resulting from multiple and extended conjugation. The band occurs in the blue-green region; hence, the dye is dark-red. The absorption band at 226 nm denotes ππ* transition resulting from conjugation, which is characteristic of benzene derivative (Finar, 2003). The absorption band shown by T. superba dye at 478 nm is attributed to ππ* transition of multiple conjugation system and it occurs in the green-blue region of the spectrum; therefore, the dye is orange in colour. The observed band at 344 nm is assigned to nπ* transition of N=O. It is noteworthy at this juncture that no dye gives a pure shade since no dye reflects only one band of wavelength (Finar, 2003). The infrared spectra of the dyes are shown in column 3 of Table 2. However, the IR spectrum of R. hispidia dye was not run due to unavailability of suitable solvent to dissolve it. The absorption band shown by P. osun at 3400 cm-1 has been attributed to the presence ofO-H (hydrogen bonded) in phenols (Furniss et al., 1989) and N-H (stretching) in secondary aromatic amines, which is hydrogen bonded. The band at 1350 to 1280 cm-1 shows the presence of C-N (stretching) in secondary amines (Furniss et al., 1989; Silveretein et al., 2005). While the absorption bands of C - O (stretching) in phenols are observed at 1350 and 1260 to 1000 cm-1. These bands result from interaction between O-H bending and C-O stretching (Furniss et al., 1989; Silveretein et al., 2005). Other bands have been assigned as indicated in Table 2. The T. superba dye showed an absorption band  at  3380 cm-1 which is attributed to O-H stretching in phenols that is hydrogen bonded (Furniss et al., 1989). The band at 2930 cm-1 is due to C-H stretching in aromatic nuclei whereas the bands at 1580 and 1440 cm-1 are C= C stretching in aromatics. The bands at 1440 and 1310 cm-1 are also attributed to -NO2 vibrations in aromatic nitro compounds whereas the band at 1310 cm-1 could also be due to C- O in phenols (Furniss et al., 1989; Silveretein et al., 2005). The observed band at 820 cm-1 could be due to C-N stretching in aromatic nitro compounds. It could also be due to 1,4-disubstitution in aromatics. However, it should be noted that the interaction of the NO2 out-of-plane and ring C-H out-of-plane bending frequencies destroys the reliability of the substitution pattern observed for nitro aromatics in the longer wavelength region of the spectrum (Silveretein et al., 2005).

 

 

A summary of the dyeing properties of the dyes is shown in Tables 3 and 4. The R. hispidia dye was not able to colour any of the fabrics. This is due to the inability of air to oxidize the reduced dye; hence, the original blue dye was not regenerated in the fabrics as expected. The dyed fabrics showed that nylon and cottonhave the greatest affinity for P. osun and T. superba dyes. This is an indication that both dyes are direct dyes for nylon and cotton.

 

 

 

 

 

 

 


 CONCLUSION

Although the structure of the dyes could not be elucidated due to lack of NMR data, the results from the chemical and spectral analyses gave vital information about the structural constituents of the dyes. The chemical analysis and the characteristic absorption in the ultraviolet and visible regions coupled with the solubility in alkaline sodium hydrosulphite give credence that the R. hispidia dye is an indigo dye (Finar, 2003) and contains O=C-C=C-C=O and –NHR as chromophore and auxochrome respectively. The results from chemical and spectral analyses also show that the P. osun dye is made up of a chromophore which is a conjugated system and auxochromes which are hydroxyl (-OH) and secondary amine (-NHR) groups. The results also show that there is presence of nitro (-NO2) group, conjugated system and hydroxyl group in the T. suberba dye. However, there is need to elucidate the exact structure of the dyes through other spectra analysis such as NMR and Mass spectroscopy.

 


 CONFLICT OF INTERESTS

The authors have not declared any conflict of interests.

 



 REFERENCES

Abrahart EN (1977). Dyes and their Intermediates. New York: Chemical Publishing pp. 1–12.

 

Akoegninou A, Van der Burg WJ, Van der Maesen LJG (2006) Flore analytique du Bénin. Edition Wageningen University papers 1:624-631.

 

Akpuaka MA (1992). Book of Abstracts, Chemical Society of Nigeria, Enugu Chapter, 6th Annual Conference pp. 13-19.

 

Akpuaka MA, Ezenwoye OM, Okerulu IO (2001). Sourcing a Yellow Dye from Turmeric (Curculonga) – A locally available rhizome, Journal of Chemical Society of Nigeria 26(1):11-13.

 

Ahon MG, Akapo-Akue JM, Kra MA, Ackah JB, Zirihi NG (2011) Antifungal activity of the aqueous and hydro-alcoholic extracts of T. superbaEngl& Diels on the in vitro growth of clinical isolates of pathogenic fungi. Agriculture and Biology Journal of North America 2:250-257.
Crossref

 

Anago EAA (2009). Activitésantibactériennes de quelquesplantes de la pharmacopéeAfricainesur des souches de E. coli productrices de bêtalactamases. Thèse de Doctorat en Biochimie à l'Universitéd' Abomey-calavi pp. 91-103.

 

Antai AB, Anaele BA, Etta KM (2005). Hypoglycaemic actions of a medicinal herb, Rothmannia hispida in diabetic rats. Mary Slessor Journal of Medicine 5(2):21-24.

 

Antai AB, Ndep AO, Etta KM (1995). Acute effects of a medicinal herb, Rothmannia hispida on alloxan-induced diabetes in the rat. Proceeding of the Annual Scientific Conference of the Nigerian Physiological Association 11(1-2): 68.

 

Antai AB, Ofem OE, Nwosu OJ, Ukafia SO, Iyadi KC, Nia R (2010). comparative effect of rothmannia hispida leaves extract and protamine-zinc insulin on alloxan-induced diabetic rats. Journal of Diabetes and Endocrinology 1(4):52-58

 

Avwioro OG, Aloamaka PC, Ojianya NU, Oduola T, Ekpo EO (2005). Extracts of Pterocarpus osun as a histological stain for collagen fibres. African Journal of Biotechnology 4(5):460-462.

 

Bahmani M, Shirzad H, Majlesi M, Shahinfard N, Rafieian-Kopaei M (2014). A review study on analgesic applications of Iranian medicinal plants. Asian Pacific journal of tropical medicine 7:S43-53.
Crossref

 

Dweck AC (2009). Comprehensive focus on natural dyes. Colour Cosmetics pp. 57-69.

 

Ezeokonkwo MA, Okoro UC (2012). New dyes for petroleum products. Journal of Emerging Trends in Engineering and Applied Sciences 3(1):8-11.

 

Finar L (2003). Organic Chemistry, Pearson Education (Singapore), Pte. Ltd.,India, 6th ed., 1: pp. 868, 873, 876, 895.

 

Furniss BS, Hannaford AJ, Smith PWG, Tatchell AR (1989). New edition Vegel's Textbook of practical Organic Chemistry, Pearson Education Ltd, England, 5th ed., pp. 141, 286, 293, 1196-1258.

 

Garcia F, Cruz-Remes L (1993). The natural xanthophylls pigment industry. Presented at the First International Symposium of Natural Colorants for Food, Nutraceuticals, Beverages and Confectionary, Amherst, MA, Nov 7-10, 57.

 

Grover N, Patni V (2011). Extraction and application of natural dye preparations from the floral parts of Woodfordia fruticosa (Linn.) Kurz. Indian Journal of Natural Products and Resources 2(4):403-408.

 

Gürses A, Acikyildiz M, Günes K, Gürses MS (2016). Dyes and pigments VIII, SpringerBrief in Green Chemistry for sustainability, pp. 83, 23. 

 

Jansen PC, Cardon D (2005). Plant Resuorses of Tropical Africa 3: Dyes and Tannins, PROTA Foundation/ Backhuys Publishers, CTA Wageningen, Nitherlands P 138.

 

Kamel MM, El-Shishtawy, RM, Yussef BM, Mashaly H (2005). Ultrasonic assisted dyeing III. Dyeing of wool with lac as a natural dye, Dyes and Pigments 65(2):103-110.
Crossref

 

Krishnaveni KS, Rao JV (2000). A New Isoflavoneglucoside from Pterocarpus santalinus, Journal of Asian Natural products Research 2:219-223.
Crossref

 

Meghmani Dyes and Intermediates Ltd. (2011). Petroleum dyes,

View

 

Morrison K (2015). The Ecology center. 

View

 

Mukherjee PK, Maiti K, Mukherjee K, Houghton P (2006). Leads from Indian Medicinal Plants with Hypoglycemic Potentials. Journal of Ethnopharmacology 106, 1-28. 
Crossref

 

Neuwinger HD (2000). African Traditional Medicine: A Dictionary of Plant Use and Applications. Stuttgart: Editions Medpharm Scientific Publishers.

 

Nnabugwu MA, Okoro UC (2012). New Dyes for Petroleum Products. Journal of Emerging Trends in Engineering and Applied Sciences 3(1):8-11.

 

Okafor SN, Obonga W, Ezeokonkwo MA, Nurudeen J, Orovwigho U, Ahiabuike J (2016). Assessment of the Health implications of Synthetic and Natural Food Colourants - A Critical Review, UK Journal of Pharmaceutical and Biosciences 4(4):01-11.

 

Oparah EN, Adamu JA, Giwa A (2014). Dyeing potential of curcuma lsonga (turme ric) on chrome - tanned leather. Nigerian Journal of Scientific Research 13(2).

 

Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S (2009). Agroforestree Database:a tree reference and selection guide, version 4.0. 

 

Pius MU, Takem LP, Ufot UF, Antai AB, Owu DU (2016). Insulin and alpha amylase levels in alloxan-induced diabetic rats and the effect of Rothmannia hispida (K. Schum) Fagerl leaf extract. The Journal of Phytopharmacology 5(1):1-5.

 

Rostad CE (2010). Analysis of solvent dyes in refined petroleum products by electrospray ionization mass spectrometry. Fuel 89 (5):997-1005.
Crossref

 

Saki K, Bahmani M, Rafieian-Kopaei M (2014). The effect of most important medicinal plants on two important psychiatric disorders (anxiety and depression)-a review. Asian Pacific Journal of Tropical Medicine 7:S34-42.
Crossref

 

Semwal RU, Semwal DK, Kapoor P (2012). Dyeing Properties of Berberis aristata DC with Natural and Synthetic Mordants. Trends in Applied Sciences Research 7:392-399. DOI: 10.3923/tasr.2012.392.399
Crossref

 

Shobayo BI, Ojo DA, Agboola DA (2015). Antibacterial Activity of Pterocarpus osun L. on Multi-Drug Resistant (MDR) Escherichia colifrom Wound Infections in Abeokuta, South-West Nigeria, Open Access Library Journal 2:1434.
Crossref

 

Silverstein RMF, Webster X, Kiemle DJ (2005). Spectrometric identification of organic compounds, 7th ed., John Wiley & Sons, Inc. USA pp. 88, 102, 104.

 

Vankar PSR, Shanker SD, Mahantab D, Tiwaric SC (2007). Characterisation of the colorants from leaves of Bischofia Javanica. International Dyer 192(3):31-37.

 

Wansi JD, Lalemand MC, Chiozem DD, Toze FAA, Mbaze LM (2007). Alpha - glucosidase inhibitory constituents from stem bark of Terminalia superba (Combretaceae). Phytochemistry Elsevier 68:2096-2100.
Crossref

 




          */?>