African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2227

Full Length Research Paper

In vivo skin irritation potential of a cream containing Moringa oleifera leaf extract

Atif Ali1*, Naveed Akhtar1, Ahmad Mahmood Mumtaz2, Muhammad Shoaib Khan1, Furqan Muhammad Iqbal3 and Syed Sauod Zaidi4
1Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan 2College of Pharmacy, G. C. University, Faisalabad, Pakistan 3Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan 4South Dakota State University, Brookings SD, USA.
Email: [email protected]

  •  Accepted: 27 September 2012
  •  Published: 15 February 2013


The aim of the present study was to evaluate the skin irritation potential of a cream containingMoringa oleifera leaf extract. Skin irritation potential of a cream containing M. oleifera leaf extract (3%) versus base was investigated by performing an in-vivo visual scoring (skin irritation), patch test and erythema index for long term study by using non-invasive instrumental assessment Mexameter in 11 volunteers in a single blinded study. The active cream and base were applied twice daily to the face (cheeks) for a period of 12 weeks. Theinstrumental measurements were carried out under a draught-free room, with controlled temperature (18.0 to 20.6°C) and relative humidity (55 to 65%). No serious adverse effects were observed. The M. oleifera leaf extract cream was not-irritant according to 48 h semi-occluded patch test. There was a significant decrease in skin erythema and base showed insignificant results when applied ANOVA. The results suggested that the M. oleifera leaf extract cream was very well accepted by all volunteers and decreased erythema content. Additionally, product can be regarded as safe for topical application.


Key words: Moringa oleifera, extract, cream, skin irritation potential, mexameter.