African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2162

Full Length Research Paper

Assessment of Cassia siamea stem bark extracts toxicity and stability in time of aqueous extract analgesic activity

Nsonde Ntandou G. F.*
  • Nsonde Ntandou G. F.*
  • Laboratoire de Biochimie et Pharmacologie, Faculté des Sciences de la Santé, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo.
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Bassoueka D. J.
  • Bassoueka D. J.
  • Laboratoire de Biochimie et Pharmacologie, Faculté des Sciences de la Santé, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo.
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Banzouzi J. T.
  • Banzouzi J. T.
  • Centre d’Etude et de Recherche Médecins d’Afrique (CERMA), B.P. 45, Brazzaville, Congo.
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Etou Ossibi A. W.
  • Etou Ossibi A. W.
  • Laboratoire de Biochimie et Pharmacologie, Faculté des Sciences de la Santé, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo.
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Elion Itou R. D. G.
  • Elion Itou R. D. G.
  • Laboratoire de Biochimie et Pharmacologie, Faculté des Sciences de la Santé, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo.
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Makambila M. C.
  • Makambila M. C.
  • Centre d’Etude et de Recherche Médecins d’Afrique (CERMA), B.P. 45, Brazzaville, Congo.
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Ramos S.
  • Ramos S.
  • Laboratoire de Pharmacologie, Centre d’Etudes sur les Ressources Végétales (CERVE), B.P.1249, Brazzaville, Congo.
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Benoit-Vical F.
  • Benoit-Vical F.
  • Service de Parasitologie et Mycologie, Hôpital de Rangueil, CHU Toulouse, 1 Avenue Jean Poulhes, TSA 50032, 31059 Toulouse Cedex 9, France.,Laboratoire de Chimie de Coordination (LCC-CNRS), 205 Route de Narbonne, 31077 Toulouse Cedex 4, France.
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Abena A. A.
  • Abena A. A.
  • Laboratoire de Biochimie et Pharmacologie, Faculté des Sciences de la Santé, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo.
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Ouamba J. M.
  • Ouamba J. M.
  • Laboratoire de Biochimie et Pharmacologie, Faculté des Sciences de la Santé, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo.
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  •  Received: 09 May 2009
  •  Accepted: 13 August 2015
  •  Published: 08 November 2015

Abstract

Cassia siamea Lam (Fabaceae), widely used in tropical countries for its nutritional and pharmacological properties, has been toxicologically evaluated. Oral administration of aqueous (CSE4) and ethanol (CSE3) Cassia siamea stem bark extracts did not show mortality up to 3000 mg/kg in albinos Wistar rats. LD 50 of petroleum ether (CSE1) and dichloromethane (CSE2) extracts were estimated at 1300 and 1275 mg/kg respectively. None of the extracts has cytotoxicity activity on KB and Vero cell lines. There were no differences in body weight and macroscopic examinations. Haematocrit, creatinine, alkaline phosphatase (ALP), alanine amino transferase (ALT) and Asparatate Amino Transferase (AST) parameters did not show difference of levels. Contrariwise, a significant increase in animal weight and blood glucose were observed in animals treated group. This increase can be explained by the presence of polysaccharides and phytosterols (stigmasterol, beta-sitosterol) in this plant. The lyophilised aqueous extract keeps its analgesic property for over two years. 
 
Key words: Cassia siamea, stem bark extracts, cytoxicity, subchronic toxicity, pharmacological stability, analgesic activity.