African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2207

Full Length Research Paper

Study of the self-association of amoxicillin, thiamine and the hetero-association with biologically active compound chlorgenic acid

Ataklti Abraha
  • Ataklti Abraha
  • Department of Physics, School of Natural and Computational Sciences, Samara University, P. O. Box 132, Samara, Ethiopia.
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Alemu Kebede
  • Alemu Kebede
  • Department of Physics, School of Natural Sciences, Adama Science and Technology University, P. O. Box 1888, Adama, Ethiopia.
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Abebe Belay
  • Abebe Belay
  • Department of Physics, School of Natural Sciences, Adama Science and Technology University, P. O. Box 1888, Adama, Ethiopia.
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  •  Received: 30 January 2015
  •  Accepted: 25 February 2016
  •  Published: 15 May 2016

Abstract

The self-association of amoxicillin (AMX) and its hetero-association with biologically active compound, chlorogenic acid (CGA) were investigated at room temperature (295°K). The dimerization constant of amoxicillin and thiamine (THIA) analyzed using the dimer model at the wavelength of 278 and 256 nm were found to be  and , respectively. The hetero-association constant of amoxicillin and chlorogenic acid analyzed using Benesi-Hildebrand approach were . Thermodynamic parameters such as Gibbs free energy, enthalpy and entropy of dimerization reactions for the self-association and hetero-association of the compounds were also investigated using Vant’s Hoff equation at the temperature ranges (295 to 305°K). The change of enthalpy calculated for amoxicillin, thiamine and the complexes of amoxicillin-chlorogenic acid are 34.73±2.17, 54.1±4.585, and 6.988±0.493  at the temperature of 305°K, respectively. The values of change in enthalpy and entropy indicate that the hydrophobic interaction play the major role in the interaction between the molecules.

 

Key words: Amoxicillin, thiamine, chlorogenic acid, UV-Vis spectroscopy, thermodynamic, self-association, hetero-association.