The self-association of amoxicillin (AMX) and its hetero-association with biologically active compound, chlorogenic acid (CGA) were investigated at room temperature (295°K). The dimerization constant of amoxicillin and thiamine (THIA) analyzed using the dimer model at the wavelength of 278 and 256 nm were found to be and , respectively. The hetero-association constant of amoxicillin and chlorogenic acid analyzed using Benesi-Hildebrand approach were . Thermodynamic parameters such as Gibbs free energy, enthalpy and entropy of dimerization reactions for the self-association and hetero-association of the compounds were also investigated using Vant’s Hoff equation at the temperature ranges (295 to 305°K). The change of enthalpy calculated for amoxicillin, thiamine and the complexes of amoxicillin-chlorogenic acid are 34.73±2.17, 54.1±4.585, and 6.988±0.493 at the temperature of 305°K, respectively. The values of change in enthalpy and entropy indicate that the hydrophobic interaction play the major role in the interaction between the molecules.
Key words: Amoxicillin, thiamine, chlorogenic acid, UV-Vis spectroscopy, thermodynamic, self-association, hetero-association.
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