African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2268

Full Length Research Paper

Rapid and sensitive fluorometric analysis of novel galactosylated cationic liposome interaction with siRNA

Shantal Dorasamy, Moganavelli Singh and Mario Ariatti*
Department of Biochemistry, Westville campus, University of KwaZulu-Natal, Durban, South Africa
Email: [email protected]

  •  Accepted: 07 November 2009
  •  Published: 31 December 2009

Abstract

RNA interference (RNAi) is being harnessed for application in the gene knockdown approach to the treatment of disease conditions. Targeted delivery of small interfering RNA (siRNA) is however an important consideration for application of this technology. We report here on the preparation of two new hepatocyte-directed liposomes designed for this purpose, containing the cholesteryl cytofectins 3β[N-(N’, N’-dimethylaminopropane)-carbamoyl] cholesterol (Chol-T) and 3β[N-(N’, N’, N’-trimethylammonium propane)-carbamoyl] cholesterol iodide (Chol-Q). Further we describe a simple yet sensitive fluorometric assay based on the displacement of siRNA-bound SYBR Green, to monitor the association of the duplex RNA with cationic liposomes under the conditions of high dilution encountered in transfection experiments.

 

Key words: siRNA, cationic liposomes, SYBR green, displacement assay.