African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2207

Full Length Research Paper

A simple and sensitive method for the detection of “Oxytetracycine” levels in ready-to-eat beef by liquid chromatography-mass spectrometry

Frida Mgonja
  • Frida Mgonja
  • Faculty of Veterinary Medicine, Sokoine University, P.O. Box 3015 Morogoro, Tanzania.
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Resto Mosha
  • Resto Mosha
  • Faculty of Veterinary Medicine, Sokoine University, P.O. Box 3015 Morogoro, Tanzania.
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Faith Mabiki
  • Faith Mabiki
  • Faculty of Sciences, Sokoine University, P.O. Box 3038 Morogoro, Tanzania.
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Kennedy Choongo
  • Kennedy Choongo
  • School of Veterinary Medicine, University of Zambia, P.O. Box 32379 Lusaka, Zambia.
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  •  Received: 02 March 2016
  •  Accepted: 14 June 2016
  •  Published: 29 July 2016

Abstract

Antimicrobial drug residues have emerged as one of the public health problems worldwide. In this study, a modified sensitive liquid chromatography mass spectrometry (LC-MS) method to detect the “Oxytetracycine” (OTC) levels in ready-to-eat beef meat in Tanzania was evaluated. Beef samples were extracted in acetonitrile in ethylenediaminetetraacetic acid (EDTA) buffer (pH 4), followed by cleaning up with Supelclean ENVI-carb active coal and a stream of nitrogen gas. The wavelength of the diode array detector (DAD) was set at 275 and 355 nm.  The detection limit of the method was calculated as 18.2 ng/g and the recovery rate of OTC was 78.6%. A total of 45 ready-to-eat beef meat samples were analyzed, with 16 (35.5%) and 29 (64.5%) barbequed and boiled samples, respectively. Of the 45 samples, 35 (77.8%) samples had OTC residues while 9 (25.7%) samples had violative residue levels above the maximum residue limits recommended by the Food and Agriculture Organization and the World Health Organization. The highest concentration was 545.2 ng/g. Therefore, withdrawal period and proper use of antibiotics for animal production should be of concern as consumers are at risk of adverse effects due to consumption of unacceptable levels of drug residues and a risk of developing microbial resistance. To the best knowledge of the authors, this is the first study to evaluate LC-MS method to detect the OTC levels in ready-to-eat beef meat in Tanzania.

Key words: Oxytetracycline, high performance liquid chromatography, mass spectrometry, ready-to-eat beef meat, residues.