African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2162

Full Length Research Paper

Salidroside decrease expression of hypoxia-inducible factor-1α in cultured human lung cancer cells

Bo Li1, Danhua Qu2, Zhongan Wang4 and Rui Xin1, 3*
1Departments of Radiology, The Second Affiliated Hospital of Jilin University, Changchun, Jilin 130021, China. 2Department of Respiratory Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin 130021, China. 3MH Radiobiology Research Unit, Jilin University School of Public Health, China. 4The Shijitan Affiliated Hospital of Capital Medical University, Beijing, China.
Email: [email protected]

  •  Accepted: 23 August 2012
  •  Published: 15 September 2012

Abstract

Salidroside is a active constituent of traditional herbal medicine Rhodiola rosea, responsible for the neuroprotective, antidepressant, anxiolytic and anti-tumor actions. However, the mechanism of its anticancer effect is unclear as there have little molecular studies regardingcell migration, invasion, cell cycle, induction of apoptosis in healthy and cancerous cells. Hypoxia-inducible factor-1α (HIF-α) plays an important role in tumor growth, angiogenesis and lymph node metastasis of non-small cell lung cancer (NSCLC). Thus, the present study aims to investigate changes from hypoxia-inducible factor-1α (HIF-α) of NSCLC A549 cell after using salidroside treatment by quantitative polymerase chain reaction (QPCR) and western-bolt on mRNA level and protein level, respectively. The results demonstrated that HIF-α was significantly down-regulated in salidroside treatment group in comparison to the A549 groupon mRNA level (P < 0.05), on protein level, HIF-α was also highly decreased in salidrosidetreatment group in comparison to control group. The present study provides improvement in understanding the molecular pathogenic mechanism of lung cancer and development salidroside as effective anticancer drugs.

 

Key words: Salidroside, A549,   HIF-α, QPCR,  NSCLC, Western-bolt.

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