Salidroside is a active constituent of traditional herbal medicine Rhodiola rosea, responsible for the neuroprotective, antidepressant, anxiolytic and anti-tumor actions. However, the mechanism of its anticancer effect is unclear as there have little molecular studies regardingcell migration, invasion, cell cycle, induction of apoptosis in healthy and cancerous cells. Hypoxia-inducible factor-1α (HIF-α) plays an important role in tumor growth, angiogenesis and lymph node metastasis of non-small cell lung cancer (NSCLC). Thus, the present study aims to investigate changes from hypoxia-inducible factor-1α (HIF-α) of NSCLC A549 cell after using salidroside treatment by quantitative polymerase chain reaction (QPCR) and western-bolt on mRNA level and protein level, respectively. The results demonstrated that HIF-α was significantly down-regulated in salidroside treatment group in comparison to the A549 groupon mRNA level (P < 0.05), on protein level, HIF-α was also highly decreased in salidrosidetreatment group in comparison to control group. The present study provides improvement in understanding the molecular pathogenic mechanism of lung cancer and development salidroside as effective anticancer drugs.
Key words: Salidroside, A549, HIF-α, QPCR, NSCLC, Western-bolt.
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