Abstract

A liquid chromatography method was developed and validated for the simultaneous determination of gatifloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations. Optimum separation was achieved in less than 5 min using a C₁₈column (250 × 4.6 mm i.d, 5 µ particle size) by isocratic elution. UV detection was carried out at 254 nm. Developed method was economical in terms of the time taken and amount of solvent consumed for each analysis. It was also validated with respect to linearity, limit of detection, limit of quantification, precision, accuracy, specificity, robustness and system suitability. The limits of detection for gatifloxacin and dexamethasone sodium phosphate were 0.397 and 0.11 µg/ml, respectively. Limits of quantification were found to be 1.203 and 0.342 µg/ml for gatifloxacin and dexamethasone sodium phosphate, respectively. The developed method was successfully applied to the simultaneous determination of gatifloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations.

Key words: Gatifloxacin, dexamethasone sodium phosphate, high performance liquid chromatography (HPLC), isocratic elution.