African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2194

Full Length Research Paper

In- vivo and vitro bioactivities of the essential oil of Duguetia lanceolata branches

Orlando Vieira de Sousa*
  • Orlando Vieira de Sousa*
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Glauciemar Del-Vechio-Vieira
  • Glauciemar Del-Vechio-Vieira
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Bruna Celeida Silva Santos
  • Bruna Celeida Silva Santos
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
C├ęlia Hitomi Yamamoto
  • C├ęlia Hitomi Yamamoto
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
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Ana L├║cia Santos de Matos Ara├║jo
  • Ana L├║cia Santos de Matos Ara├║jo
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Ailson da Luz Andr├ę de Ara├║jo
  • Ailson da Luz Andr├ę de Ara├║jo
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Miriam Aparecida de Oliveira Pinto
  • Miriam Aparecida de Oliveira Pinto
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Mirian Pereira Rodarte
  • Mirian Pereira Rodarte
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Maria Silvana Alves
  • Maria Silvana Alves
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universit├írio, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
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  •  Received: 25 November 2015
  •  Accepted: 29 January 2016
  •  Published: 15 April 2016

Abstract

The present study investigated the in- vivo and vitro bioactivities of the essential oil of Duguetia lanceolata branches (EODL) and caryophyllene oxide (CO). The chemical composition of EODL was analyzed by GC and GC/MS. Acetic acid-induced writhing, formalin, hot plate and tail-immersion methods were used to evaluate the antinociceptive effect in mice. Anti-inflammatory activity was tested by carrageenan-induced paw edema and pleurisy in rats, and in mouse models of acute ear inflammation induced by croton oil and arachidonic acid. 2,2-Diphenyl-1-picrylhydrazyl (DPPH), reducing power and ­ŁŤŻ-carotene bleaching assays were used to determine the antioxidant bioactivity. ´üó-Elemene, caryophyllene oxide and ´üó-eudesmol were the major components found in EODL. When tested, EODL and CO reduced the number of writhes and both phases of the time of paw licking, while the reaction time on a hot plate and the pain latency duration by the tail-immersion assay were increased. EODL and CO were effective against the paw edema, exudate volume, leucocyte migration and ear edema. EODL and CO also showed in vitro antioxidant activity. These results suggest that caryophyllene oxide is one of the active compounds found in EODL and could partially justify the bioactivity of this oil.

Key words: Duguetia lanceolata, essential oil, caryophyllene oxide, antinociceptive activity, anti-inflammatory activity, antioxidant activity.