African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Camellia sinensis extract inhibits in vitro pancreatic lipase and has preventive effect on obesity in female rat fed a high-fat diet

Erika Carolina Vieira-Almeida*
  • Erika Carolina Vieira-Almeida*
  • Biotechnology Department, Federal University of Tocantins, Rua Badejós, Lote 7, Chácaras 69/72, Zona Rural. CEP: 77402-970, Brazil.
  • Google Scholar
Acácio Antonio Pigoso
  • Acácio Antonio Pigoso
  • Pharmacy Department, Hermínio Ometto Foundation, FHO – UNIARARAS, Avenida Dr. Maximiliano Baruto, 500, Jardim Universitário, Araras, SP, CEP: 13607-339, Brazil.
  • Google Scholar
Alex Fernando de Almeida
  • Alex Fernando de Almeida
  • Bioprocess Engineering and Biotechnology - Federal University of Tocantins, Rua Badejós, Lote 7, Chácaras 69/72, Zona Rural. CEP: 77402-970, Brazil.
  • Google Scholar


  •  Received: 18 March 2015
  •  Accepted: 13 August 2015
  •  Published: 08 October 2015

Abstract

The aim of this study was to evaluate eight plants extract for porcine pancreatic lipase inhibition, characterizing biochemically the extract with high inhibitory activity and its effects on preventing weight gain in female rat fed a high-fat diet (HFD). In vitro pancreatic lipase inhibition was carried out in ρ-nitrophenyl-laurate substrates and a double-reciprocal plot was used for inhibition mechanism identification. In vivo experiments, female rats were fed with a standard diet or high fat diet (HFD), HFD+orlistat, HFD+22.5 mg/ml GT, and HFD+112.5 mg/ml GT. Feed intake, weight body gain, fecal lipid excretion and biochemistry parameters were analyzed. Camellia sinensis extract had the highest inhibitory lipase activity (76.65 ± 2.04%) with a non-competitive inhibition. C. sinensis administration, equivalent to 112.5 mg/ml, promoted weight loss, while 22.5 mg/ml increased fecal excretion of lipids in 31.41%. C. sinensis extract is certainly a promising alternative for preventive obesity treatment, since biochemical parameters analyzed showed significantly, reduction in the serum triglycerides levels and significantly decreased the LDL-cholesterol basal levels when compared with animals that did not receive a fat diet.

 

Key words: Enzyme inhibition, plant extracts, animal, preventive obesity.