African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Antioxidant and anticholinesterase activities of the essential oil of Eugenia dysenterica DC.

Chistiane Mendes Feitosa*
  • Chistiane Mendes Feitosa*
  • Department of Chemistry, Federal University of Piaui, ‘Campus Universitário Ministro Petrônio Portela’, Bloco 06-Bairro Ininga,64049-550, Teresina, PI, Brazil.
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Alisson dos Reis Barbosa
  • Alisson dos Reis Barbosa
  • Department of Chemistry, Federal University of Piaui, ‘Campus Universitário Ministro Petrônio Portela’, Bloco 06-Bairro Ininga,64049-550, Teresina, PI, Brazil.
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Cassio Herbert Santos de Melo
  • Cassio Herbert Santos de Melo
  • Department of Chemistry, Federal University of Piaui, ‘Campus Universitário Ministro Petrônio Portela’, Bloco 06-Bairro Ininga,64049-550, Teresina, PI, Brazil.
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Rivelilson Mendes Freitas
  • Rivelilson Mendes Freitas
  • Department of Chemistry, Federal University of Piaui, ‘Campus Universitário Ministro Petrônio Portela’, Bloco 06-Bairro Ininga,64049-550, Teresina, PI, Brazil.
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Jose Eraldo do Nascimento Fontes
  • Jose Eraldo do Nascimento Fontes
  • Chemistry Institute, University of Campinas (UNICAMP), CP 6154, 13083-970, Campinas, SP, Brazil.
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Emmanoel Vilaca Costa
  • Emmanoel Vilaca Costa
  • Department of Chemistry, Federal University of Amazonas, Bloco 08, Setor Norte do Campus Universitário Sen. Arthur Virgílio Filho, Av. Rodrigo Otávio, 6.200, Coroado, 69077-000 Manaus, AM, Brazil.
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Khaled Nabih Zaki Rashed
  • Khaled Nabih Zaki Rashed
  • National Research Centre, Pharmacognosy Department, Dokki, Giza, Egypt.
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Joaquim Soares da Costa Junior
  • Joaquim Soares da Costa Junior
  • Department of Teacher Education, Arts and Sciences Federal Institute of Piauí, Praça da Liberdade n° 1597, Centro Teresina Piauí Brazil.
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  •  Received: 28 August 2015
  •  Accepted: 05 November 2015
  •  Published: 22 May 2017

Abstract

This paper describes the anticholinesterase and antioxidant activities of Eugenia dysenterica DC. (O. Berg. (Myrtaceae) essential oils from leaves (EOED). EOED were obtained by hydrodistillation using a Clevenger-type apparatus and the products were analyzed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID). The main constituents of EOED were caryophyllene oxide (66.3%), isoledene (3.9%), 1,3,8-p-menthatriene (3.5%), mustakone (3.46%), β-phellandrene (1.7%), and selin-11-en-4-α-ol (1.7%). The antioxidant assay was performed based on the formation of thiobarbituric acid reactive substances (TBARS), hydroxyl radical, and nitric oxide production. By performing the Ellman assay, it was observed that EOED was able to inhibit the enzyme acetylcholinesterase (AChE) with an IC50 = 0.92 g.ml-1 promising better value when compared with the drug rivastigmine (IC50 = 1.87 g.ml-1), used in the treatment of Alzheimer's disease. The caryophyllene oxide (the main compound) was tested after purification on the AChE with an IC50 = 0.31 g.ml-1. Caryophyllene oxide (the majority compound) was tested on the AChE and presented the IC50 = 0.31 g.ml-1. At concentrations of 0.9, 1.8, 3.6, 5.4, and 7.2 g.ml-1, it was found out that EOED prevented lipid peroxidation inhibiting amount of TBARS formed in a similar manner to ascorbic acid. In addition, there was a reduction in the production of hydroxyl radical as well as the production of nitric oxide. To the best of our knowledge, this is the first report on compounds from   this species that have activity for potentially preventing neurodegenerative disorders.

Key words: Eugenia dysenterica, essential oil, antioxidant, anticholinesterase.