African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Role of gastrointestinal motility/gastric emptying in cisplatin-induced vomiting in pigeon

Ihsan Ullah1*, Fazal Subhan1, Khalid Rauf 2, Amir Badshah1 and Gowhar Ali1
1Department of Pharmacy, University of Peshawar, Peshawar, Pakistan. 2Department of Pharmacy, COMSATS institute of information technology, Abbottabad, KPK, Pakistan.
Email: [email protected]

  •  Accepted: 07 August 2012
  •  Published: 22 September 2012

Abstract

In this study we screened various crude fractions of Cannabis sativa indigenous to Pakistan against cisplatin-induced vomiting in pigeon and to find the involvement of gastrointestinal (GIT) motility and gastric emptying in said complication. Cannabis sativa hexane (CS-HexFr 5, 10 and 15 mg/kg), n-butanol (CS-ButFr 5 and 10 mg/kg) and methanol fractions (CS-MetFr 10 and 15 mg/kg) were examined against cisplatin-induced vomiting and their possible effects on GIT motility/gastric emptying in pigeon by charcoal propulsion method. Standard prokinetic drugs metoclopramide (MCP, 30 mg/kg) and carbachol (0.1 mg/kg) were screened in combination with CS-HexFr to estimate the involvement of gastric emptying and GIT motility in cisplatin-induced vomiting. CS-HexFr at the dose of 10 mg/kg once and twice daily attenuated cisplatin-induced vomiting up to 55.45 and 68.86% (P < 0.01), respectively. CS-MetFr and CS-ButFr failed to reduce cisplatin-induced vomiting (P > 0.05). CS-HexFr 10 mg/kg caused up to 26.62% suppression in GIT motility as compared to vehicle treatment group. MCP (30 mg/kg) and carbachol (0.1 mg/kg) completely antagonized the suppression caused by CS-HexFr 10 mg and in combination enhanced the antiemetic profile at 12 to 24 h, while no enhanced activity was observed at 0 to 12 h. The findings suggest that the decrease in GIT motility/gastric emptying is playing a role at least in part in the vomiting induced by cisplatin commencing just after the peak vomiting response of acute phase in pigeon.

 

Key words: Cisplatin, vomiting, gastrointestinal motility, Cannabis sativa, carbachol.