African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2227

Full Length Research Paper

Puerarin inhibits acute nociceptive responses via the P2X3 receptor in rat dorsal root ganglia

Changshui Xu
  • Changshui Xu
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Bo Fan, Shicheng Yu
  • Bo Fan, Shicheng Yu
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Shuangmei Liu
  • Shuangmei Liu
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Huangui Xiong
  • Huangui Xiong
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Yun Gao, Guilin Li
  • Yun Gao, Guilin Li
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Hong Xu
  • Hong Xu
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Xiaoli Tang
  • Xiaoli Tang
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Qicheng Zhu
  • Qicheng Zhu
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Chaoran Zheng
  • Chaoran Zheng
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Bing Wu
  • Bing Wu
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Lichao Peng
  • Lichao Peng
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Miaomiao Song
  • Miaomiao Song
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Qin Wu
  • Qin Wu
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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Shangdong Liang
  • Shangdong Liang
  • Department of Physiology, Medical College of Nanchang University, Nanchang 330006, P.R. China.
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  •  Accepted: 20 January 2014
  •  Published: 29 January 2014

Abstract

The effects of puerarin on acute nociception mediated by the P2X3 receptor in rat dorsal root ganglia (DRG) were studied. Intrathecal injection of adenosine triphosphate (ATP) or α,β-methylene-ATP (α,β-meATP) could potentiate acute nociceptive responses induced by intraplantar injection of ATP or α,β-meATP in rat hindpaw. Intraplantar injection of formalin increased the expression of P2X3 in rat DRG and induced acute nociceptive responses. These induced nociceptive responses were inhibited by intrathecal injection of puerarin, which also decreased the up-regulated expression of P2X3 mRNA and protein in DRG induced by intraplantar injection of formalin. Molecular docking studies revealed that puerarin could interact perfectly with homology-modeled rat P2X3 receptor (rP2X3). rP2X3 can be restricted to binding ATP because of its combination with puerarin (increasing the concentration of free ATP), therefore blocking the rP2X3 channel. Whole cell patch clamp recording showed that puerarin inhibited the potentiation of P2X3 receptor-mediated currents induced by lipopolysaccharide. These results demonstrated that puerarin inhibited nociceptive responses induced by ATP, α,β-meATP, or formalin and its inhibitory effect was mediated by reduction of inflammatory pain-induced up-regulation of P2X3 receptor expression and blockade of ATP binding sites on the P2X3 receptor. Our results suggest that puerarin could decrease acute pain mediated by the P2X3 receptor in the DRG.

Key words: Puerarin, acute nociceptive response, P2X3 receptor, dorsal root ganglia, whole cell patch clamp.