African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2148

Full Length Research Paper

Oral administration of Qing-Shu-Yi-Qi-Tang reduce lung cancer-induced cachexia in mice

Yen-Jung Chou1, Cheng-Yi Kuo4, Pei-Yi Chen1, Lih-Lian Chen1, Kun-Yun Yeh2, Hsien-Shen Kuo4 and Chin-Hsien Chang1,2,3*
1Center for Traditional Chinese Medicine, En Chu Kong Hospital No. 399, Fuxing Rd., Sanxia District, New Taipei City 23702, Taiwan. 2Division of Hemato-Oncology, Department of Internal Medicine, Keelung Chang Gung Memorial Hospital, No. 222, Mai-Jin Road, Keelung, Taiwan. 3School of Traditional Chinese Medicine, Chang Gung University, No. 259, Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan County, Taiwan. 4Seeing BioScience Co Ltd., 12F, No. 126, Sec. 1, San-Ming Rd., Banqiao District, New Taipei City, Taiwan.
Email: [email protected]

  •  Accepted: 05 October 2011
  •  Published: 15 January 2012

Abstract

This study is to investigate anti-inflammatory and anti-cachectic effect of Qing-Shu-Yi-Qi-Tang (QSYQT) in RAW 264.7 cells and Lewis lung cancer cells bearing mice. We examined the effect of QSYQT on LPS-induced inflammatory activity in a murine macrophage cell line, Raw 264.7. We evaluated cachectic parameters, such as weight loss, food intake and tumor size in tumor-bearing and non-tumor-bearing mice treated with QSYQT decoction or a normal diet. Cytokine production in cell culture and in cachectic mice was quantified by ELISA. NF-kB expression was measured using real-time polymerase chain reaction (PCR). QSYQT significantly reduced the IL-1β, IL-6 and TNF-α production in LPS-stimulated RAW 264.7 cells. Treatment of QSYQT prevented weight loss in tumor-bearing mice without affecting food intake or tumor growth. Furthermore, the level of proinflammatory cytokines, IL-1β, IL-6 and TNF-α in sera were significantly reduced in tumor-bearing mice treated with QSYQT. NF-kB expression in spleen of LLC-derived mice was decreased in the presence of QSYQT. Our results revealed that QSYQT exerts an anti-cachectic effect on LLC-induced cachectic mice. The effect is conclusively associated with modulation of IL-6 production through NF-kB.

 

Key words: Chinese traditional medicine, cachexia, anti-inflammation, IL-6, NF-kB, palliative medicine.

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