African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 718

Full Length Research Paper

In vitro potential antimicrobial activities of medicinal plants, Cirsium arvense and Cirsium japonicum

Amjad Khan
  • Amjad Khan
  • Department of Animal Science, Quaid-e-azame University, Islamabad, Pakistan.
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Zia Ul Haq Khan*
  • Zia Ul Haq Khan*
  • School of Science, Beijing University of Chemical Technology, Beijing, 100029, China.
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Pingyu Wan
  • Pingyu Wan
  • School of Science, Beijing University of Chemical Technology, Beijing, 100029, China.
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Yongmei Chen
  • Yongmei Chen
  • School of Science, Beijing University of Chemical Technology, Beijing, 100029, China.
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Don Don Kong
  • Don Don Kong
  • School of Science, Beijing University of Chemical Technology, Beijing, 100029, China.
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Mo Heng Liang
  • Mo Heng Liang
  • School of Science, Beijing University of Chemical Technology, Beijing, 100029, China.
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Karman Tahir
  • Karman Tahir
  • School of Science, Beijing University of Chemical Technology, Beijing, 100029, China.
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Arif Ullah Khan
  • Arif Ullah Khan
  • School of Life Science, Beijing University of Chemical Technology, Beijing, 100029, China.
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Sadia Nazir
  • Sadia Nazir
  • Department of Chemistry, Gomal University, DIK, KPK, Pakistan.
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Shafi Ullah Khan
  • Shafi Ullah Khan
  • Department of Chemistry, Gomal University, DIK, KPK, Pakistan.
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  •  Received: 04 November 2013
  •  Accepted: 05 February 2014
  •  Published: 28 February 2014

Abstract

Antimicrobial activities of Cirsium species (Cirsium arvense and cirsium japonicum) was evaluated against six bacterial species, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa (clinical strain/PIMS), Enterobacter (clinical strain/PIMS), Staphylococcus aureus (MRSA, clinical strain/PIMS) and six fungus Trichophyton longifusus, Candida albicans, Aspergillus flavus, Microsporum canis, Candida glaberata and Fusarium solani. The methanolic extracts of these plants were fractionated with n-hexane to get (F1), chloroform (F2), ethyl acetate(F3), n-butanol(F4) and methanolic fraction (F5). The agar well diffusion assay and agar dilution susceptibility testing were carried out to determine the zone of inhibitions and the minimum inhibitory concentration, respectively. Ager well diffusion methods was used for antifungal activity. The zones of inhibition were determined using standard agent. The entire fraction showed some activity against these pathogens, but F2 of both plants shows very good activity. The present study explains the potential antimicrobial properties of C. arvense and C. japonicum. From these observations, it is clear that Cirsium species contain antimicrobial constituents.

Key words: Traditional medicine, crude extract, antimicrobial activities, Cirsium arvense, cirsium japonicum.