Leaf segments of Stevia were cultured on MS medium supplemented with varying concentrations (0.5, 1.0, 1.5, 2.0, 2.5 mg/L-1) of growth regulators (BAP and 2, 4-D). Ninety one percent aseptic cultures were obtained when sterilized with 0.1% HgCl2 for 10 min. The highest amount of callus was obtained in MS medium supplemented with1.0 mg/L-1 BAP+0.5 mg/L-1 2, 4-D, respectively. On the other hand, 2.5 mg/L-1 BAP + 0.5 2,4-D showed lowest performance. Highest shoot was obtained in 2.0 mg/L-1 BAP. These shoots was transfer into different concentration of IBA, NAA and IAA (0.5. 1.0, 1.5, and 2.0) used. Highest rooting percentage was recorded on MS medium with 0.5 mg/L-1 IAA. The rooted plantlets were transfer into mist chamber with relative humidity for 2 - 4 week after these plants were hardened and successfully established in soil.
Key words: Stevia rebaudiana, sweetener, callus, leaf.
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