African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 718

Short Communication

An efficient protocol for in vitro clonal propagation of natural sweetener plant (Stevia rebaudiana Bertoni)

Ashish Ojha1*, V. N. Sharma1 and Vinay Sharma2
  1Department of Biotechnology, Plant Tissue Culture and Research Development Laboratory, MIET group of Institutions, Meerut- 250005, U. P. India. 2Department of Biotechnology and Bioscience, Banasthali University, Banasthali, Rajasthan, India.
Email: [email protected]

  •  Accepted: 18 May 2010
  •  Published: 31 August 2010



Leaf segments of Stevia were cultured on MS medium supplemented with varying concentrations (0.5, 1.0, 1.5, 2.0, 2.5 mg/L-1) of growth regulators (BAP and 2, 4-D). Ninety one percent aseptic cultures were obtained when sterilized with 0.1% HgCl2 for 10 min. The highest amount of callus was obtained in MS medium supplemented with1.0 mg/L-1 BAP+0.5 mg/L-1 2, 4-D, respectively. On the other hand, 2.5 mg/L-1 BAP + 0.5 2,4-D showed lowest performance. Highest shoot was obtained in 2.0 mg/L-1 BAP. These shoots was transfer into different concentration of IBA, NAA and IAA (0.5. 1.0, 1.5, and 2.0) used. Highest rooting percentage was recorded on MS medium with 0.5 mg/L-1 IAA. The rooted plantlets were transfer into mist chamber with relative humidity for 2 - 4 week after these plants were hardened and successfully established in soil.


Key words: Stevia rebaudiana, sweetener, callus, leaf.