African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 686

Full Length Research Paper

Identity and genetic diversity of Pectobacterium spp., causal agents of potato soft rot in Zanjan, Iran

E. Tavasoli1, A. R. Marefat2 and N. Hassanzadeh1*
1Department of Plant Pathology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of Plant Pathology, Faculty of Agriculture, Zanjan University, Zanjan, Iran.
Email: [email protected]

  • Article Number - 2C7C45D9680
  • Vol.5(6), pp. 329-336, June 2011
  •  Accepted: 09 February 2011
  •  Published: 30 June 2011

Abstract

Zanjan province is one of the areas of potato production in Iran with more than 6917 ha of cultivation. One of the most important factors to limit potato cultivation is soft rot bacteria belonging to the family Enterobacteriaceae. During the years 2007 to 2008, different potato fields and the most important potato storages in Zanjan province were surveyed. Phenotypic identification of the strains was performed using recommended biochemical and physiological tests. Genetic diversity was determined by BOX-PCR and ERIC-PCR. Also all of the strains were identified with specific primers (Y1, Y2 andECA1f, ECA2r). The strains belonged to "carotovora" group and identified asPectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum. Although there were some atypical characteristics among the strains, but a very close relationship were found between DNA fingerprints and geographical origins of isolates.

 

Key words: Pectobacterium, Soft rot, Black-leg, BOX-PCR, ERIC-PCR.

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