The present study was conducted to establish a protocol for in vitro propagation of an endemic medicinal plant Ceropegia juncea (Asclepiadaceae) from one month old aseptic seedling nodal explants. The highest shoot multiplication rate of 20.65 ± 0.20 shoots/explant was achieved on Murashige and Skoog medium supplemented with BAP 8.87 mM + TDZ 4.54 mM. Excised shoots were rooted on half strength MS medium with IBA 4.90 mM + NAA 1.27 mM. 78% of the rooted shoots survived in the field.
Key words: Ceropegia juncea, in vitro propagation, endemic medicinal plant, nodal explants, multiple shoots.
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