African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 718

Full Length Research Paper

Microtuberization of Ceropegia spiralis Wight and Ceropegia pusilla Wt. and Arn.

K. Sri Rama Murthy1*, R. Kondamudi1 and S. Karuppusamy2        
1School of Conservation Biology and Plant Biotechnology, Department of Biotechnology, Montessori Mahila Kalasala, Vijayawada, Andhra Pradesh, -520 010, India. 2Department of Botany, the Madura College, Madurai, Tamil Nadu - 625 011, India.
Email: [email protected]

  •  Accepted: 11 August 2011
  •  Published: 30 September 2012

Abstract

The ability of Ceropegia spiralis Wight and Ceropegia pusilla Wight and Arn. to form microtubers in vitro was studied, to conserve these endemic and endangered species. Murashige and Skoog’s (MS) medium supplemented with various concentrations of cytokinins and auxins supported induction of two types of microtubers, that is, basal and aerial tubers. The nodal explants of C. spiralis best tuberized on MS medium supplemented with 6-benzylaminopurine (BAP) 13.32 µM + Naphthalene Acetic Acid (NAA) 1.34 to 2.68 µM. The aerial tubers were observed on Indole-3-Butyric Acid (IBA) 49.2 µM + 6% sucrose and Indol-3-Acetic Acid (IAA) 11.54 µM + 3% sucrose augmented ½ MS media. In another case, single roots were tuberized on ½ MS medium supplemented with IAA 11.54 µM + 3% sucrose in C. spiralis. Whereas, in C. pusilla, the medium containing BAP 13.32µM + 2, 4-D 0.11 µM + 3% sucrose played major role in the induction of basal tubers. Strength of the medium, plant growth regulators (PGRs) concentration and additives played an effective role in the formation of microtubers. If these valuable ornamental and medicinal plants were conserved, we may add another flower to the bouquet and a new drug as well.

 

Key words: Aerial tubers, basal tubers, Ceropegia spiralis, C. pusilla, single root tubers, thin cell layers.