African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 762

Full Length Research Paper

Genotypic screening of tomato’s AREB 1 gene for drought tolerance and computational protein structure prediction

Ja’afar Umar
  • Ja’afar Umar
  • Department of Biology, Faculty of Science, Federal University Birnin Kebbi, Nigeria.
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Adamu Aliyu Aliero
  • Adamu Aliyu Aliero
  • Department of Biological Sciences, Faculty of Science, Usmanu Danfodiyo University Sokoto, Nigeria.
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Kasimu Shehu
  • Kasimu Shehu
  • Department of Biology, Faculty of Science, Federal University Birnin Kebbi, Nigeria.
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Obadiah Caleb Dikko
  • Obadiah Caleb Dikko
  • Department of Biological Sciences, Faculty of Science, Usmanu Danfodiyo University Sokoto, Nigeria.
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  •  Received: 25 December 2020
  •  Accepted: 02 March 2021
  •  Published: 30 June 2021

Abstract

In this investigation, the coding sequence of the drought-stress inducible gene AREB1 in tomato derived from cDNA indicated 100% identity with the reference gene in the NCBI PlantEnsembl database. The protein structure of the AREB1 sequence derived from polymerase chain reaction from tomato DNA template was done using ExPASy and its protein parameter tools ProtParam. The structures of AREB1 protein showed a MolProbity score of 1.49. Multiple sequence alignment of AREB1 gene from 20 tomato genotypes revealed a phylogenetic tree with five clusters, each with the same evolutionary trend. The nucleotide sequence analysis showed higher similarities among the selected tomato genotypes. This indicated the conserved nature of the gene among the genotypes.

 

Key words: Tomato, drought, resistant, AREB1gene, AREB1protein.