A protocol was developed by in vitro regeneration by shoot induction of Cucumis anguria, a plant used as medicine and vegetable belonging to the family Cucurbitaceae. High frequencies of multiple shoot regeneration were achieved from nodal explants on MS fortified with 2 mg/l 6-benzyl amino purine (BAP) and 0.5 mg/l indole acetic acid (IAA). Five to ten shoots per explant were obtained. The elongated shoots were sub cultured for rooting on MS supplemented with 2 mg/l napthalin acetic acid (NAA). The in vitro raised plantlets were acclimated in green house and successfully transplanted to natural condition with 75% survival.
Key words: In vitro regeneration, Cucumis anguria, shoot tip explants, MS medium.
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