African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 718

Full Length Research Paper

Direct regeneration of the medicinal herb Cucumis anguria L from shoot tip explants

S. Senthil Kumar
PG and Research Department of Botany, Vivekanandha  College of Arts and Sciences For Women,  Tiruchengode,Tiruchirappalli 620 002, Tamil Nadu, India
Email: [email protected]

  •  Accepted: 16 September 2013
  •  Published: 31 October 2013

Abstract

A protocol was developed by in vitro regeneration by shoot induction of Cucumis anguria, a plant used as medicine and vegetable belonging to the family Cucurbitaceae. High frequencies of multiple shoot regeneration were achieved from nodal explants on MS fortified with 2 mg/l 6-benzyl amino purine (BAP) and 0.5 mg/l indole acetic acid (IAA). Five to ten shoots per explant were obtained. The elongated shoots were sub cultured for rooting on MS supplemented with 2 mg/l napthalin acetic acid (NAA). The in vitro raised plantlets were acclimated in green house and successfully transplanted to natural condition with 75% survival.

 

Key words: In vitro regeneration, Cucumis anguria, shoot tip explants, MS medium.