African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 683

Full Length Research Paper

Effects of different hormones on organogenesis in vitro of some varieties of cassava (Manihot esculenta CRANTZ) grown in Senegal

Abdoulaye FAYE
  • Abdoulaye FAYE
  • Laboratory Campus of Plant Biotechnology, Faculty of Sciences and Technology, University Cheikh Anta Diop of Dakar, BP: 5005 Dakar-Fann, Senegal.
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Maurice SAGNA
  • Maurice SAGNA
  • Laboratory Campus of Plant Biotechnology, Faculty of Sciences and Technology, University Cheikh Anta Diop of Dakar, BP: 5005 Dakar-Fann, Senegal.
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Papa Demba KANE
  • Papa Demba KANE
  • Senegalese Institute of Agricultural Research (ISRA), BP: 3120 Dakar, Senegal.
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Djibril SANE
  • Djibril SANE
  • Laboratory Campus of Plant Biotechnology, Faculty of Sciences and Technology, University Cheikh Anta Diop of Dakar, BP: 5005 Dakar-Fann, Senegal.
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  •  Received: 12 November 2014
  •  Accepted: 13 July 2015
  •  Published: 30 August 2015

Abstract

Cassava (Manihot esculenta) is a perennial euphorbiaceous shrub grown mainly for its starchy tubers and its leaves rich in protein. The most known method of propagation of this crop is the classical cuttings’ planting. However, in vitro propagation appears most useful and permits to obtain high quantity of healthy vegetable material in a short period. In this work, it was to study the impact of different hormones on the organogenetic capacities in vitro of five cassava varieties cultivated in Senegal. Axillary uninodal sections were disinfected and cultured in Murashige and Skoog (MS) basal medium added or not of different concentrations (0.1, 0.5 and 1 mg/L) of auxin (α-naphthalene acetic acid (NAA)) or cytokines (benzyl aminopurine (BAP) and kinetin). Best shoot growth and rooting was observed in MS medium containing 0.1 mg/L kinetin with normal development of the leaves. Highest proliferation of shoots and leaves was obtained with medium MS + BAP 1 mg/L. Callus formation was observed in all media containing hormone but most in MS + NAA 1 mg/L. This work proposes a rapid and economic technique for cassava multiplication.
 
Key words: Organogenesis in vitro, cassava, varieties, hormones, Senegal.