African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 757

Full Length Research Paper

Phytochemical analysis of Ziziphus mucronata Willd. extract and screening for antifungal activity against peanut pathogens

Kadidia Koïta
  • Kadidia Koïta
  • Department of Biology and Plant Physiology, Biosciences Laboratory, University Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Fidele Bawomon Neya
  • Fidele Bawomon Neya
  • Department of Biology and Plant Physiology, Biosciences Laboratory, University Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Nelson Opoku
  • Nelson Opoku
  • Faculty of Agricultural, University for Development Studies, Tamale, Ghana.
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Yves Baissac
  • Yves Baissac
  • Laboratory of Biochemistry and Plant Physiology, University of Montpellier II, F-34095 Montpellier Cedex 05, France.
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Claudine Campa
  • Claudine Campa
  • IRD, UMR IPME (Interactions Plantes-Microorganismes-Environnement), 911 Avenue Agropolis, BP 64501 34394, Montpellier cedex 5, France.
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Philippe Sankara
  • Philippe Sankara
  • Department of Biology and Plant Physiology, Biosciences Laboratory, University Ouaga I Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 25 August 2017
  •  Accepted: 12 October 2017
  •  Published: 30 November 2017

Abstract

Chemical analysis of aqueous extract of Ziziphus mucronata Willd. was determined by liquid chromatography–mass spectrometry (LC-MS) analysis. Among the 11 compounds found, catechin, rutin (quercetin 3-O-rutinoside), delphinidin-3-glucoside, isoquercetin (hyperoside) and quercitrin (quercetine3,7-O-L-dirhanmopyranoside) were identified as the major phenolics components in this aqueous plant extract. To elute the target compounds, the fractionation of crude extract was carried out on solid phase extraction (SPE) columns. The different fractions (from FZ1 to FZ5) obtained after fractionation were evaluated in vitro against economically important foliar fungal pathogens of peanut, including Cercospora arachidicola, Phaeoisariopsis personata and Puccinia arachidis. The treatments with Z. mucronata fractions were compared with negative control (water) and standard solutions of cathechin and rutin (1 mg/mL). All the fractions recorded an inhibitory effect, firstly on conidial germination and germ tube elongation, secondly on disease evolution on peanut leaves previously inoculated by fungi; the level of efficiency of inhibition varied from 40.55 (FZ1 against C. arachidicola) to 57.14% (FZ2 and FZ3 against P. arachidis). Then, spores of P. arachidis seemed to be more sensitive to the treatment.

 

Key words: Fungal pathogens, peanut, plant extract, phenolics, Ziziphus mucronata Willd.