An experiment was conducted to determine the physiological and cytological changes in carrot (Daucus carota L.) due to infection of cucumber mosaic virus. The virus isolate causing mottling was detected by reverse transcriptase polymerase chain reaction (RT-PCR) by showing amplification of the expected size of 650 base pair (bp)deoxyribonucleic acid (DNA) band. Regarding the physiological changes, carbon, nitrogen and protein contents was decreased in infected plants as compared to healthy plants while phosphorous content was increased in diseased plant. In cytological changes, the scattered metaphase was observed in the diseased plant cells. Mitotic index of the diseased cells was decreased while N/C ratio was increased. Chromatin bridges were also observed at anaphase I and II due to infection of cucumber mosaic virus.
Key words: Physiological changes, cytological changes, Daucus carota.
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