Roses are one of the world's most important ornamentals for a long time and are most often used for ornamental, medicinal and aromatic purposes. A method for the proliferation and growth of rose (Rosa miniature) was developed. First to seventh nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS) containing several concentrations of Benzyl amino purine (BAP) and indol butyric acid (IBA). Multiple shoot formation of up to 4 shoots from a single node was obtained on MS medium supplemented with 2 mgl-1 BAP and 0.1 mgl-1 IBA. The mean length of shoots were 38 mm. The mean number of leaves 13 and color of leaves red-green were observed. The regenerated shoot readily rooted on as the same MS medium with growth regulators, mean length of root were 33 mm. In vitroflowering will observe on rose plants cultured.
Key word: Proliferation, Rosa miniature, ornamental, rooting.
BAP, Benzyl amino purine; IBA, indol butyric acid.
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