The aim of this research work was to decide the event and look at the circulation of Tomato spotted wilt virus (TSWV) and Cucumber mosaic virus (CMV) contaminating tomato plants developed in southwestern Nigeria. 200 and seventy-five tomato leaf tests were gathered from chosen cultivates in 16 significant tomato delivering zones spread over the six conditions of Southwest Nigeria. Side effects saw on the ranches visited included leaf mosaic, bending, mottling, wilting, twisting, yellowing, purpling, filiform and corruption.
Triple counteracting agent sandwich compound connected immunosorbent examine (TAS-ELISA) procedure was utilized to decide the nearness of TSWV. All examples tried negative to the TSWV antibodies. In the interim, when these examples were tried for the nearness of Cucumber mosaic virus (CMV) utilizing the antigen-covered plate ELISA method, the outcome uncovered that 53 examples (19.27 %) of chose tests were CMV positive.
For the atomic corroborative test for TSWV, 30 examples with the most noteworthy TAS-ELISA readings were exposed to add up to RNA extraction followed by turn around translation polymerase chain response (RT-PCR) utilizing three unique sets of preliminaries explicit for the intensification of TSWV nucleocapsid protein (N) quality. The result of the RT-PCR atomic test affirms the nonattendance of TSWV in the examples. RT-PCR was additionally directed utilizing preliminaries CMV1/CMV2 on 50 delegate tests which were arbitrarily chosen. The aftereffect of the RT-PCR corroborative test for CMV displayed the normal ~500 base pair amplicon groups in just the CMV seropositive examples. Nucleotide grouping investigation of the amplicons uncovered 97-99 % nucleotide arrangement homologies with relating successions of detailed CMV strains accessible in the Genebank in this manner affirming the nearness of CMV in tomato leaf tests.
This investigation presumed that TSWV is definitely not a significant risk to tomato creation in southwestern Nigeria while CMV has risen as one of the significant tomato pathogens in the area
Keywords: Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), Tomato, Southwestern Nigeria