Clinical Reviews and Opinions

  • Abbreviation: Clin. Rev. Opinions
  • Language: English
  • ISSN: 2141-2553
  • DOI: 10.5897/CRO
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

The effects of combined vitamin C and E in streptozotocin-induced diabetic rat kidney

Aysel Kukner
  • Aysel Kukner
  • Abant Izzet Baysal University Faculty of Medicine, Department of Histology and Embryology, 14280 Bolu-Turkey.
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Neriman Colakoglu
  • Neriman Colakoglu
  • Fırat University, Faculty of Medicine, Department of Histology and Embryology, Turkey.
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Candan Ozogul
  • Candan Ozogul
  • Gazi University, Faculty of Medicine, Department of Histology and Embryology, Turkey.
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Mustafa Naziroglu
  • Mustafa Naziroglu
  • Süleyman Demirel University, Faculty of Medicine, Department of Biophysics, Turkey.
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Tulin Firat
  • Tulin Firat
  • Abant Izzet Baysal University Faculty of Medicine, Department of Histology and Embryology, 14280 Bolu-Turkey.
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  •  Accepted: 23 October 2009
  •  Published: 30 November 2009

Abstract

Diabetic nephropathy is a serious complication of diabetes mellitus. Oxidative stress has been suggested to play a key role in the pathogenesis of diabetic nephropathy. Vitamins C and E play important roles in the antioxidant defense system. It is likely that both vitamins act in a synergistic manner, with vitamin E primarily being oxidized to the tocopheroxyl radical and then reduced back to tocopherol by vitamin C. The purpose of this study was to determine the effects of supplementation of vitamins C and E (VCE) on diabetic rat kidney. Adult female Wistar rats were used in the study. The animals were divided into three groups. Group I: the control group; Group II: diabetic group, streptozotocin (40 mg/kg) was administered group; Group III: diabetes+VCE group, received a diet containing a combination of ascorbic acid and di-α-tocopheryl acetate per kg of feed. Rats were killed on day 21 and renal tissues were taken and fixed in 2.5% glutaraldehyde solution for electron microscopic examination. When compared with the control group, congestion of the glomerular capillaries, increased mesangial cells and distinct mesangium, shortened podocyte processes and disappearance of filtration slit pore of diabetic rat kidney were observed. In the group treated with VCE, glomerular changes were less distinct than the diabetic group. Lengths of the pedicles were similar to the control group. In conclusion, VCE reduced the changes in the glomerular structures due to diabetes.

 

Key words: Diabetes, kidney, vitamin C, vitamin E, ultrastructure.