International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 100

Full Length Research Paper

Proposal for access to the femur in rats

Clayton Miguel Costa1, Geraldo Bernardes1, Sandro Melim Sgrott1, Jorge Bins Ely1, Luismar Marques Porto2 and Armando José d’Acampora1*
1Department of Surgery, Federal University of Santa Catarina, UFSC, Brazil. 2Graduate Program in Chemical Engineering, Federal University of Santa Catarina, UFSC, Brazil.
Email: [email protected]

  •  Accepted: 11 March 2011
  •  Published: 30 April 2011

Abstract

Reconstructions, implantation procedures and bone synthesis materials used to treat bone loss caused by tumors or fractures require in vivo testing. In this study, we created an access route to the femur in rats, allowing us to conduct in vivo bone experiments with less cost, more easily obtainable materials and simplified storage and care. Eighteen male Wistar rats, 180 days of age and weighting 350 to 370 g were surgically operated. Their femurs were accessed and exposed quickly and safely. This proved to be an effective method for experiments in which the bone needs to be approached and studied. The approach proposed in this study is suitable for full exposure of the rat femur.

Key words: Rats, femur, models, animal.