International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 100

Full Length Research Paper

Nutrigenomic analysis of mulberry silkworm (Bombyx mori L.) strains using polymerase chain reaction - simple sequence repeats (PCR-SSR)

Chinnaswamy Ramesha1*, Savarapu Sugnana Kumari2, Chebba Moremnagari Anuradha3, Hothur Lakshmi4 and Chitta Suresh Kumar5
1Silkworm Breeding and Molecular Genetics Laboratory, Andhra Pradesh State Sericulture   Research and Development Institute (APSSRDI), Kirikera-515 211, Hindupur, AP, India. 2Biology Division, Indian Institute of Chemical Technology, Tarnaka, Hyderabad - 500 007, AP, India. 3Department of Biotechnology, College of Engineering and Technology, Sri Krishnadevaraya University, Anantapur-515 003, AP, India. 4Silkworm Genetics and Breeding Laboratory, Central Sericultural Research and Training, Institute (CSR and TI), Central Silk Board, Berhmpore - 742 101, West Bengal, India. 5Bioinformatics Centre, Department of Biochemistry, Sri Krishnadevaraya University, Anantapur- 515 003, AP, India.  
Email: [email protected]

  •  Accepted: 05 October 2010
  •  Published: 31 December 2010

Abstract

The DNA marker use in assisting selection are safe method in breeding process and it is an important tool for authentication of new gene cascade in genome. In mulberry silkworm, the major economic and nutrigenomic traits are polygenic in nature. In the present study, we have utilized ten PCR-SSR microsatellite markers to gain better understanding on genotyping of certain nutrigenomic gene loci in nutritionally efficient silkworm breeds / hybrids. Results showed that  a single yet varying size amplified band in all four parental silkworm strains (RMG4, RMW2, RBD1 and RBO2) and two clear amplified bands in the hybrids (RMG4 × RBD1 and RMW2 × RBO2) with different molecular weight from three PCR-SSR primers loci viz., F11139, F10429 and F10705. The PCR-SSR results demonstrated that homozygosity in newly evaluated nutritionally efficient parental silkworm strains and heterozygosity in hybrid. These investigations authentically confirmed the previous findings of heterotic nutritionally efficient silkworm hybrids with superior nutrigenomic traits. The developed molecular analysis in silkworm could be utilized for the benefit of the farmers in sericulture industry. In conclusion, these results would be useful in identification of nutrigenomic cascade of genes in silkworm and also emphasize the future prospects of silkworm functional mechanism in nutrigenomic studies.

Key words: Silkworm, breed, hybrid, nutrigenomics, PCR-SSR marker, homozygosity, heterozygosity, cascade of genes.