International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 99

Full Length Research Paper

In vitro effects of gibberellic acid and sucrose concentration on micropropagation of two elite sweet potato cultivars in Rwanda

Valery Ndagijimana
  • Valery Ndagijimana
  • Faculty of Agriculture, National University of Rwanda (NUR), P. O. Box117, Huye, Rwanda.
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Jane Kahia*
  • Jane Kahia*
  • World Agroforestry Centre (ICRAF) Cote d’ Ivoire Country Program Cocody Mermoz, Abidjan, Côte d’Ivoire | 08 BP 2823 ABIDJAN 08.
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Theodore Asiimwe
  • Theodore Asiimwe
  • Rwanda Agriculture Board (RAB), P. O. Box 5016. Kigali, Rwanda.
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Peter Yao Sallah
  • Peter Yao Sallah
  • Faculty of Agriculture, National University of Rwanda (NUR), P. O. Box117, Huye, Rwanda.
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Bancy Waweru
  • Bancy Waweru
  • Rwanda Agriculture Board (RAB), P. O. Box 5016. Kigali, Rwanda.
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Isidore Mushimiyimana
  • Isidore Mushimiyimana
  • Rwanda Agriculture Board (RAB), P. O. Box 5016. Kigali, Rwanda.
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Jean Ndirigwe
  • Jean Ndirigwe
  • Rwanda Agriculture Board (RAB), P. O. Box 5016. Kigali, Rwanda.
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Sindi Kirimi
  • Sindi Kirimi
  • International Potato Center (CIP), Sub-Saharan Africa Region, P. O. Box 25171, Nairobi, Kenya
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Damien Shumbusha
  • Damien Shumbusha
  • Rwanda Agriculture Board (RAB), P. O. Box 5016. Kigali, Rwanda.
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Peter Njenga
  • Peter Njenga
  • Jomo Kenyatta University of Agriculture and Technology, Box 62000, Nairobi, Kenya.
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Modeste Kouassi
  • Modeste Kouassi
  • Centre National de Recherche Agronomique (CNRA), Laboratoire Central de Biotechnologies (LCB), 01 BP 1740 Abidjan 01, Côte d’Ivoire.
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Edmond Koffi
  • Edmond Koffi
  • Centre National de Recherche Agronomique (CNRA), Laboratoire Central de Biotechnologies (LCB), 01 BP 1740 Abidjan 01, Côte d’Ivoire.
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  •  Received: 22 November 2013
  •  Accepted: 29 March 2014
  •  Published: 30 April 2014

Abstract

The current study aimed at evaluating the effect of gibberellic acid (GA3) and sucrose on in vitro propagation of two elite sweet potato cultivars (Ukerewe and Gihingamukungu). Nodal explants from in vitro growing plantlets were harvested and cultured on Murashige and Skoog media supplemented with 2.5, 5, 10, 20 and 40 μM, GA3. In a separate experiment, sucrose was evaluated at 30, 60, 90, 120, 150, 180 and 210 mM. For Ukerewe, the explants cultured on medium supplemented with 10 µM GA3 recorded the longest (2.78 ± 0.36 cm) microshoots.. On the other hand, cultivar Gihingamukungu explants cultured on media supplemented with 2.5 GA3 µM produced the longest ((3.23 ± 0.40 cm) microshoots. Nodal explants from the two cultivars cultured on media supplemented with sucrose 150 mM yielded the longest microshoots (2.51 ± 0.26 and 2.34 ± 0.24 cm, respectively). From the results of the current study, it can be concluded that for micropropagation of the cultivar Ukerewe 10 µM GA3 should be used while 2.5 GA3 µM should be used for micropropagtion of cultivar Gihingamukungu. The regenerated plantlets were successfully weaned in the greenhouse. The protocol developed in this research will open new prospects for massive propagation of the elite sweet potato cultivars in Rwanda.

 

Key words: Ukerewe, Gihingamukungu, nodal explants, microshoot.