International Journal of
Fisheries and Aquaculture

  • Abbreviation: Int. J. Fish. Aquac.
  • Language: English
  • ISSN: 2006-9839
  • DOI: 10.5897/IJFA
  • Start Year: 2010
  • Published Articles: 223

Full Length Research Paper

Nitrite intoxication of Clarias gariepinus at different water temperatures

Ajani Funmilola1* and Adeyemo Olanike Kudirat2
1Department of Animal Science and Fisheries Management, Bowen University, lwo, Osun State, Nigeria 2Department of Veterinary Public Health and Preventive Medicine, University of lbadan, lbadan, Nigeria.
Email: [email protected]

  •  Accepted: 21 October 2011
  •  Published: 09 March 2012

Abstract

 

Clarias gariepinus (Burchell, 1822) of mean weight 320±11.2 g and total length 60.0±1.0 cm were exposed to nitrite (0.2 mg/l NO2) for 48 h at 27 and 35°C in order to investigate the effects of nitrite poisoning at these water temperatures. The effects of nitrite exposure on fish was assessed on selected haematological and biochemical indicators of the blood. The test was performed in a semi-static assay for 48 h. Fish were kept in thermostart –controlled water baths each containing 15 L of test solution. Four groups each containing 8 specimens of 3 month old C. gariepinus were exposed to nitrite at the different water temperatures. Nitrite exposed fish showed lower haematocrit value (PCV) at both experimental temperatures compared with controls. Significantly higher PCV values were recorded in fish with nitrite at 27°C (36.67±0.57%) when compared with fish at 35°C (33.33±0.57%). A significant difference was also recorded for haemoglobin level and the erythrocyte counts. Leucocyte count in fish with nitrite at 27C was significantly higher (P<0.05) compared to the count at 35°C. For Plasma Biochemical parameters, significant differences were observed between the 2 groups for Kconcentration (27°C, 4.9±0.10 mmol/l; 35°C, 4.1±0.12 mmol/l). Statistically significant differences were observed in plasma CI-   and Naconcentrations in fish at both temperatures. It has been established from this study that nitrite is more toxic at higher temperature.

 

Key words: Haematology, plasma biochemistry, Clarias gariepinus, temperature.