Abstract

Genetic basis of different leaf esterase and root peroxidase isozymes was investigated by analyzing their zymogram phenotypes in selfed and intercrossed progenies of two local varieties (used as control) and three induced true breeding dwarf mutant lines of grass pea (Lathyrus sativus L.). Two non-allelic genes, df1/df2 and df3 controlling dwarfism in grass pea were included in the present linkage studies with different isozyme loci. The dwarf mutants could be distinguished from one another and also from control varieties by the presence of unique allozyme/s coded by allele/s in different loci. A good fit to 1: 2: 1 in F₂ and 1:1 in back cross indicated monogenic segregation of isozyme loci with co-dominant expression of different alleles and the isozymes were functionally monomeric. Linkage analysis revealed closely linked associations among Est-1, Est-2and df3 loci and also between Est-3 and Prx-1 loci. All five genes, however, assorted independently with df1/df2 as well as with prx-3. Locus prx-2 was monomorphic in all five parents.

Key words: Dwarf mutants, isozymes, linkage, Lathyrus sativus L.