International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Morphological and molecular variation in Ethiopian lentil (Lens culinaris Medikus) varieties

Edossa Fikiru1*, Kassahun Tesfaye2 and Endashaw Bekele2
  1Madawalabu University, P. O. Box 247, Bale-Robe, Ethiopia. 2Department of Biology, Science Faculty, Addis Ababa University, P. O. Box, 1176, Addis Ababa, Ethiopia
Email: [email protected]

  •  Accepted: 19 May 2011
  •  Published: 30 May 2011

Abstract

 

Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada’a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety. Alemaya, Alemtena and Assano were early in flowering and maturity, while Chekol, EL-142 and Teshale were intermediate in flowering but early in maturity. The Euclidian distance between Ada’a and Assano was high, while the distance between Alemtena and Teshale was the least. Clustering analysis based on phenotypic data classified the varieties into two groups. ISSR analyses revealed that 75.93% of the amplified bands were polymorphic with average gene diversity () of 0.2734. Inter-varietal genetic distance between Gudo and Chekol was high, while that between Gudo and Alemaya was low. Clustering analysis based on ISSR marker also classified the varieties into two groups. The correlation (=0.334) between morphological and ISSR dissimilarity matrices was significant. Results of the present study reveal the genetic variation between Ethiopian lentil varieties which would help in selection of varieties for specific agro-ecology and/or purpose, and for further breeding activities.

 

Key words: ISSR marker, genetic diversity, lentil varieties, morphological variation, correlation.