International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Gas chromatography-mass spectrometry (GC-MS) analysis of petroleum ether extract (oil) and bio-assays of crude extract of Iris germanica

Syeda Farina Asghar1*, Habib-ur-Rehman1, M. I. Choudahry2 and Atta-ur-Rahman2
  1Department of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad-13100, Pakistan. 2Husein Ebrahim Jamal (H. E. J.) Research institute of Chemistry, University of Karachi, Karachi-75270, Pakistan.
Email: [email protected]

  •  Accepted: 22 July 2011
  •  Published: 30 August 2011


Iris plants have immense medicinal importance and are therefore used in the treatment of cancer, inflammation, bacterial and viral infections (Nadkarni, 1976). Gas chromatography-mass spectrometry analysis of the petroleum ether extract (oil) of the plant have resulted in the identification of eleven compounds, 9-hexadecanoic acid methyl ester, 9-octadecenoic acid methyl ester, 8-octadecenoic acid methyl ester, 11-octadecenoic acid methyl, 10-octadecenoic acid methyl ester, 13-octadecenoic acid methyl ester, 16-octadecenoic acid methyl ester, 1,2-benzenedicarboxylic acid diisooctyl ester, bis (2-ethylhexyl) phthalate, methyl 6-methyl heptanoate and nonanoic acid, 9-oxo-methyl ester. The bioassay of petroleum ether extract (oil) showed that, it is potent against for antimicrobial and antioxidantactivities.


Key words: Iris germanica, gas chromatography-mass spectrometry (GC-MS)  analysis, identification of compounds, bioassay