International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 131

Full Length Research Paper

Effect of genotype, explant source and medium on in vitro regeneration of tomato

Praveen Mamidala1,2 and Rama Swamy Nanna1*
  1Department of Biotechnology, Kakatiya University, Warangal, A.P- 506009, India. 2Department of Biotechnology, Vaagdevi College of Engineering, Warangal, 506009, India.
Email: [email protected]

  •  Accepted: 27 April 2011
  •  Published: 28 April 2011

Abstract

For the present investigations, five cultivars including PKM-1, Moneymaker, Microtom, Micro-MsK and White Cherry of tomato (Lycopersicon esculentum Mill.) were selected. The explants cotyledon, hypocotyl and leaf of all the cultivars of tomato were cultured on MS medium fortified with different concentrations of Benzyl amino purine (BAP) (1.0 to 3.0 mg/L)and 0.1 mg/L indole-3-acetic acid (IAA). Adventitious shoot buds were induced at the cut ends of the explants after three weeks of culture. Adventitious shoots were induced from hypocotyl, cotyledon and leaf explants on MS medium fortified with 1.0 mg/l BAP + 0.1mg/l IAA (Medium A); 2.0 mg/l BAP+0.1 mg/l IAA (Medium B) and 3.0 mg/L BAP + 0.1 mg/L IAA (Medium C). Of the five genotypes tested, PKM-1 showed highest number of shoots per explant followed by Micro-MSK, Microtom and Money maker on MS medium augmented with different concentrations of growth regulators used (A, B & C). The lowest regeneration efficiency was observed from all the explants used in White Cherry. More number of adventitious shoots was induced from leaf explants compared to cotyledon and hypocotyl explants in all the genotypes of tomato studied. Among the three media tested, medium‘B’ showed superiority in cotyledon and leaf explants compared to medium ‘A’. Whereas medium ‘C’ had shown poor response on regeneration ability of all the explants tested irrespective of genotypes. In vitro rooting was achieved on MS medium augmented with 0.1 mg/L NAA (Naphthalene acetic acid) in all the genotypes. Thus the plant regeneration was found to be influenced by the genotype, explant and type of medium.

 

Key words: Tomato, cotyledon, hypocotyl, leaf, regeneration, plantlet formation