International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 139

Full Length Research Paper

Biodiversity of uncultured bacteria in hypersaline lakes, Siwa oasis, Egypt, as determined by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes

Hosam E. Elsaied*
  • Hosam E. Elsaied*
  • Genetics and Genetic engineering research group, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
  • Google Scholar
Mohamad Saad Abd El-Karim
  • Mohamad Saad Abd El-Karim
  • Phycology research group, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
  • Google Scholar
Mai A. Wassel
  • Mai A. Wassel
  • Genetics and Genetic engineering research group, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
  • Google Scholar


  •  Received: 01 August 2016
  •  Accepted: 12 October 2016
  •  Published: 30 April 2017

References

Abd El-Karim M (2016). Chemical composition and antimicrobial activities of cyanobacterial mats from hyper saline Lakes, northern western desert, Egypt. J. Appl. Sci. 16(1):1.
Crossref

 

Abd El-Karim M, Goher M (2016). Heavy metal concentrations in cyanobacterial mats and underlying sediments in some northern western desert lakes of Egypt. J. Fish. Aquat. Sci. 11:163-173.
Crossref

 

Anton J, Oren A, Benlloch S, Rodriguez-Valera F, Amann R, Rossello-Mora R (2002). Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the bacteria from saltern crystallizer ponds. Int. J. Syst. Evol. Microbiol. 52:485-491.
Crossref

 

Anton J, Pe-a1 A, Santos F, Martínez-García M, Schmitt-Kopplin P, Rosselló-Mora, R (2008). Distribution, abundance and diversity of the extremely halophilic bacterium Salinibacter ruber. Saline Syst. 4:15.
Crossref

 

Borsodi A, Felfoldi T, Mathe I, Bognar V, Knab M, Krett G, Jurecska L, Toth E, Marialigeti K (2013). Phylogenetic diversity of bacterial and archaeal communities inhabiting the saline Lake Red located in Sovata, Romania. Extremophiles 17:87-98.
Crossref

 

Boujelben I, Yarza P, Almansa C, Villamor J, Maalej S, Anton J, Santos F (2012). Virioplankton community structure in Tunisian solar salterns. Appl. Environ. Microbiol. 78:7429-7437.
Crossref

 

Boutaiba S, Hacene H, Bidle K, Maupin-Furlow J (2011). Microbial diversity of the hypersaline Sidi Ameur and Himalatt salt lakes of the Algerian Sahara. J. Arid Environ. 75:909-916.
Crossref

 

Buerger S, Spoering A, Gavrish E, Leslin C, Ling L, Epstein S (2012). Microbial scout hypothesis and microbial discovery. Appl. Environ. Microbiol. 78:3229-3233.
Crossref

 

DasSarma S, DasSarma P (2012). Halophiles. Encycl. Life Sci. pp. 1-9.
Crossref

 

Edbeib M, Abdul Wahab R, Huyop F (2016). Halophiles: biology, adaptation, and their role in decontamination of hypersaline environments. World J. Microbiol. Biotechnol. 32:135.
Crossref

 

Elsaied H (2013). Monitoring of uncultured Dunaliella sp. in an Egyptian solar saltern field based on RuBisCO-encoding gene cbbL. Afr. J. Biotechnol. 12:5361-5369.
Crossref

 

Gugliandolo C, Lentini V, Maugeri T (2011). Distribution and diversity of bacteria in a saline meromictic lake as determined by PCR-DGGE of 16S rRNA gene fragments. Curr. Microbiol. 62:159-166.
Crossref

 

Holt J, Lewin R (1968). Herpetosiphon aurantiacus gen. et sp. n., a new filamentous gliding organism. J. Bacteriol. 95:2407-2408.

 

Keresztes Z, Felfldi T, Somogyi B, Szekely G, Dragos N, Marialigeti K, Bartha C, Voros L (2012). First record of picophytoplankton diversity in Central European hypersaline lakes. Extremophiles 16:759-769.
Crossref

 

Luque R, Bejar V, Quesada E, Llamas I (2014). Diversity of halophilic bacteria isolated from Rambla Salada, Murcia (Spain). Can. J. Microbiol. 60:839-846.
Crossref

 

Mahmoud A, Abd El-Karim M (2016). Fatty acid profiles, pigments and biochemical contents of cyanobacterial mat in some lakes of North Western Desert, Egypt. Am. J. Biochem. Mol. Biol. 6:15-24.
Crossref

 

Makhdoumi-Kakhki A, Amoozegar M, Kazemi B, Pasic L, Ventosa A (2012). Prokaryotic diversity in Aran-Bidgol Salt Lake, the largest hypersaline Playa in Iran. Microbes Environ. 27:87-93.
Crossref

 

Mehrshad M, Amoozegar MA, Makhdoumi A, Rasooli M, Asadi B, Schumann P, Ventosa A (2015). Halovarius luteus gen. nov., sp. nov., a novel extremely halophilic archaeon from Urmia salt lake. Iran. Int. J. Syst. Evol. Microbiol. 65:2420-2425.
Crossref

 

Mongodin EF, Nelson KE, Daugherty S, Deboy RT, Wister J, Khouri H, Weidman J, Walsh DA, Papke RT, Sanchez Perez G, Sharma AK, Nesbø CL, MacLeod D, Bapteste E, Doolittle WF, Charlebois RL, Legault B, Rodriguez-Valera F (2005). The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea. Proc. Natl. Acad. Sci. USA. 102:18147–18152.
Crossref

 

Moune S, Caumette P, Matheron R, Willison J (2003). Molecular sequence analysis of prokaryotic diversity in the anoxic sediments underlying cyanobacterial mats of two hypersaline ponds in Mediterranean salterns. FEMS Microbiol. Ecol. 44:117-130.
Crossref

 

Mutlu MB, GUVen K (2015). Bacterial diversity in Camalt Saltern, Turkey. Polish J. Microbiol. 64:37-45.

 

Muyzer G, De Waal EC, Uitterlinden AG (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes encoding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700.

 

Nubel U, Engelen B, Felske A, Snaidr J, Wieshuber A, Amann RI, Ludwig W Backhaus H (1996). Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. Bacteriol. 178:5636–5643.
Crossref

 

Ollivier B, Caumette P, Garcia JL, Mah RA (1994). Anaerobic bacteria from hypersaline environments. Microbiol. Rev. 58:27-38.

 

Oren A (2002). Molecular ecology of extremely halophilic archaea and bacteria. FEMS Microbiol. Ecol. 39:1-7.
Crossref

 

Pinar G, Krakova L, Pangallo D, Piombino-Mascali D, Maixner F, Zink A, Sterflinger K (2014). Halophilic bacteria are colonizing the exhibition areas of the Capuchin Catacombs in Palermo, Italy. Extremophiles18:677-691.

 

Sahl J, Pace N, Spear J (2008). Comparative molecular analysis of endoevaporitic microbial communities. Appl. Environ. Microbiol. 74:6444-6446.
Crossref

 

Sly L, Taghavi M, Fegan M (1998). Phylogenetic heterogeneity within the genus Herpetosiphon: transfer of the marine species Herpetosiphon cohaerans, Herpetosiphon nigricans and Herpetosiphon persicus to the genus Lewinella gen. nov. in the Flexibacter– Bacteroides–Cytophaga phylum. Int. J. Syst. Bacteriol. 48:731-737.
Crossref

 

Suzuki M, Giovannoni S (1996). Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl. Environ. Microbiol. 62:625-630.

 

Tazi L, Breakwell DP, Harker AR, Crandall KA (2014). Life in extreme environments: microbial diversity in Great Salt Lake. Utah. Extremophiles 18:525-535.
Crossref

 

Wei X, Jiang Y, Chen X, Jiang Y, Lai H (2015). Amycolatopsis flava sp. nov., a halophilic actinomycete isolated from Dead Sea. Antonie Van Leeuwenhoek.
Crossref

 

Xiang W, Guo J, Feng W, Huang M, Chen H, Zhao J, Zhang J, Yang Z, Sun Q (2008). Community of extremely halophilic bacteria in historic Dagong brine well in southwestern China. World J. Microbiol. Biotechnol. 24:2297-2305.
Crossref