Genetic variability, heritability and genetic advance among yield and yield related traits of advanced Tef [Eragrostis tef (Zucc.) Trotter] breeding lines
December 2023
In Ethiopia, tef is one of the most significant crops that are grown extensively as a staple cereal crop. The evaluation of genetic variability in crop species is one of the key activities in plant breeding, which supports in the creation of breeding strategies to meet a diversify objectives. A field experiment was therefore conducted to determine genetic variability, heritability, and genetic advanced for yield and...
Sequencing of S5 gene in autotetraploid rice japonica and indica to overcome F1 hybrids embryo sac sterility
July 2023
Autotetraploid rice is a new germplasm developed through diploid chromosome doubling. Hybrids developed by indica autotetraploid rice crossed by japonica autotetraploid rice has clear biological advantage on F1, causing widespread concerns for special research evolution to evaluate and utilize F1 hybrid vigor. However, the widespread fertility of F1 indica and japonica autotetraploid rice is low, which makes it...
In silico analysis of mutations associated with genetic variability of the strain African cassava mosaic virus (ACMV) in three departments of Côte d'Ivoire
January 2023
Cassava (Manihot esculenta Crantz) is an important crop that constitutes staple food and income for 800 million people worldwide. Cassava yield in Côte d'Ivoire is reduced due to a variety of factors, including cassava mosaic disease. Despite the impact of the pathogen Cassava Mosaic Virus (CMV) on production, genetic diversity of this virus is rarely studied in Côte d'Ivoire. This study aims to...
Contribution of cytogenetic and molecular biology in disorders of sex development diagnosis: About 55 cases
January 2023
Disorders of sex development (DSD) when diagnosed early is important as it pose a real public health problem in Senegal. Among the supporting tools, molecular ones, which are not available everywhere are very useful. In this context, cytogenetic and molecular analyses were implemented in cytology laboratory at the Aristide Le Dantec hospital to enhance the DSDs diagnosis as well as evaluate the impact of the...
Evaluation of growth and survival performances of Pure Borana goat and their crosses with Boer goats
March 2022
Evaluation on growth and survival performances of Borana goats and their crosses with Boer was performed at Yabello Pastoral and Dryland Agriculture Research Center. General Linear Model (GLM) and Chi-square procedure of Statistical Analysis System (SAS) were used to analyze the effect of breed group, sex, parity, birth type and kidding season on traits such as BWT, PADG, WWT, 6MWT, 9MWT, YWT, PoWADG and mortality rate....
Genetic variability of Maure zebu in Mali using SSR markers
December 2021
In Mali, Maure’s zebu breed has good dairy production abilities. Females are often used as a matrix in crosses in peri-urban areas with exotic breeds. Despite the enthusiasm of its productive abilities, the genetic diversity of this breed is not yet sufficiently assessed. In order to contribute to a better understanding of the genetic diversity of this breed, 82 animals were chosen from the farms of four districts...
ABO and Rh (D) blood group distribution among blood donors: A Study of Natural and Computational Science Faculty graduating class students at Woldia University, Ethiopia
January 2021
The ABO and Rh (D) blood type distributions vary from one ethnic group to another. However, they play an essential part in population genetics studies, blood transfusion practices and certain medico-legal cases. Thus, this study is aimed to assess ABO and Rh (D) blood group distribution patterns among Natural and Computational Science faculty graduating class students in Woldia University and to document a blood group...
Molecular characterization and in silico analysis of mutations associated with extended-spectrum beta-lactamase resistance in uropathogenic Escherichia coli and Klebisiella pneumoniae in two hospitals, Côte d'Ivoire
January 2021
Escherichia coli and Klebsiella pneumoniae are pathogens frequently involved in urinary tract infections with high epidemic potential. The increase and spread resistance of these microbes to broad spectrum beta-lactam antibiotics are usually reported and is a real public health concern in Côte d’Ivoire but information on genetic variants and intragenic mutations encoding these resistances are scarce. The aim...
Genetic typing and in silico assignment of smoked and fresh bushmeat sold on markets and restaurants in west-central Côte d'Ivoire
January 2021
Poachers and bushmeat traders most of the time modify the wildlife materials before commercialization. Therefore, the morphological identification of species is no longer possible. However, accurate identification of the sale wildlife materials is essential for species identification, quantification and better conservation management. The aim of this study is to take advantages of specific gene sequences and in silico...
Notice of Retraction: Verification of single nucleotide polymorphism (SNP) markers associated with maize (Zea mays. L) streak virus resistance in early generation maize lines
October 2020
The editors of the International Journal of Genetics and Molecular Biology (IJGMB) are retracting the article, “Verification of single nucleotide polymorphism (SNP) markers associated with maize (Zea mays. L) streak virus resistance in early generation maize lines” published in 2020 (Vol.12(1), pp. 36-45, February 2020). This is based on the corresponding author’s (Solomon Shibeshi) declaration of a...
Molecular characterization of viruses causing maize lethal necrosis disease in South-Rift region, Kenya
July 2020
The first report of Maize lethal necrosis (MLN) disease in Africa was in Bomet, Kenya, nine years ago. It has since spread in East and Central African (ECA) countries, causing massive yield losses. Currently, 90% of the preferred commercial maize germplasm grown by farmers in Kenya is susceptible to MLN disease. As such, the disease has continued to pose a serious challenge to food security in the ECA region. This study...
Impact of glutathione S-transferase genes polymorphisms on human papillomavirus infection and precancerous lesions in West African women
July 2020
Genetic polymorphisms of certain classes of glutathione S-transferase (GST), enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with risk of several cancers such as cervical cancer. The aim of this study is to investigate the impact of glutathione S-transferase M1 and T1 deletion on high-risk human papillomavirus (HR-HPV) infections and on dysplasia. A case-control study was...
Genetic analysis of twenty two selected genotypes of Jatropha curcas L. (physic nut) from Africa, Asia and America, using SSR and AFLP markers
July 2020
Twenty two genotypes of J. curcas L. from Africa (Senegal, Burkina Faso, Mali, Congo and Madagascar Island), Asia (Cambodia, China and India) and America (Ecuador, Dominican Republic and Brazil) selected for their vigor and their productivity were analyzed with ten SSR primer pairs and six AFLP primer combinations. The two marker approaches showed their ability to effectively reveal polymorphism among the selected...
Retracted: Verification of single nucleotide polymorphism (SNP) markers associated with maize (Zea mays. L) streak virus resistance in early generation maize lines
February 2020
Maize streak virus (MSV) is a devastating viral disease of maize in sub-Saharan Africa. The disease may cause up to 100% yield loss in susceptible crops. The use of molecular markers can facilitate the development of varieties resistant to the virus. The objective of this study is to assess the usefulness of Single Nucleotide Polymorphism (SNP) markers linked to MSV resistance in selecting for resistance at early...
First detection of the hemizygote frameshift variant of the DMD gene in a 13-year-old patient affected by dystrophinopathy (Duchenne DMD and Becker BMD) at Saint Camille Hospital of Ouagadougou, Burkina Faso: Case report
February 2020
Genetic diseases are poorly reported in sub-Saharan Africa, especially in Burkina Faso. The reasons for this reality are multifactorial including, the difficulty of diagnostic confirmation, financial accessibility and even the difficulty with referral of cases by medical staff. Genetic diseases, although relatively rare, exist in families and deserve special attention in our paraclinical assessments. Few cases can be...
Distribution of groundnut rosette disease and sequence diversity of groundnut rosette virus associated satellite RNA (Sat-RNA) in Western Kenya
February 2020
Production of groundnuts (Arachis hypogaea L.) in Western Kenya is mainly constrained by groundnut rosette disease (GRD) which cause up to 100% yield loss. This disease expresses different symptoms as a result of variations in the groundnut rosette virus (GRV) associated satellite-ribonucleic acid (GRV Sat-RNA). Over the past 20 years, no work had been done to document the status of the disease in Kenya. Additionally,...
Morphological diversity patterns among selected elite Shea trees (Vitellaria paradoxa C.F. Gaertn.) from Tchologo and Bagoué districts in Northern Côte d’Ivoire
February 2020
Agromorphological diversity structure of the elite shea trees identified in village lands and conserved in situ in the districts of Bagoué and Tchologo by the shea breeding program of the University of Peleforo Gon Coulibaly (UPGC, Côte d’Ivoire), are not known. In the present study, we characterized the agromorphological parameters of 220 elite shea trees using a set of 12 quantitative traits. The...
Mapping of fourteen high-risk human papillomavirus genotypes by molecular detection in sexually active women in the West African sub-region
February 2020
The aim of this study was to determine the distribution of high-risk human papillomavirus genotypes (HR-HPV) in women from the general population of five West African countries. This was a cross-sectional descriptive study, involving 2133 women from nine cities of five West African countries: Benin, Burkina Faso, Côte d'Ivoire, Niger and Togo. Women were screened for precancerous cervical lesions and HR-HPV...
Characterisation of three morphotypes of Solenostemon rotundifolius [(Poir.) J. K. Morton] cultivated in Burkina Faso using quantitative traits
December 2019
Evaluation of intra specific variability is a key step toward conservation and sustainable use of species. This study was carried out to describe the morphotypes of Solenostemon rotundifolius (Lamiaceae) based on quantitative traits. Three accessions (E02, E35 and E20), representing the morphotypes “A”, “B” and “C” were characterised in Randomised Complete Block Design with three...
Primary morphological characterization of West African dwarf (Djallonké) ewes from Côte d’Ivoire based on qualitative and quantitative traits
December 2019
Phenotypic characterization is used to identify and document diversity within and between distinct breeds, based on their observable attributes. Study to assess the body characteristics and variability of 204 Djallonké (West African dwarf) ewes was conducted in three agro-ecological zones (central, western and northern) of Côte d’Ivoire, from November 2016 to April 2017. Animals were described using...
Assessment of radio-sensitivity for three cowpea genotypes to gamma irradiation
December 2019
Cowpea (Vigna unguiculata [L.] Walp) is an important food legume. However, production is limited by various biotic and abiotic constraints in sub-Saharan Africa and reduced genetic variability hinders crop improvement efforts. Gamma ray irradiation has been used as a mutagen to create variability but optimal dose determination is a prerequisite to maximize the recovery of mutant plants. In this study, three genotypes...
Coexistence between (TOHO-type and BES-type) extended-spectrum β-lactamase genes of identified enterobacteria at Saint Camille Hospital, Ouagadougou, West Africa
December 2019
Coexistence between the TOHO-type and Brazilian extended-spectrum (BES)-type of extended-spectrum β-lactamase (ESBL)-produced by bacteria caused public health issue. Several studies have been reported on the coexistence between blaTEM, blaCTX-M and blaSHV genes in ESBL in broad spectrum enterobacteria. The present study involved the prevalence of coexistence of blaTOHO and blaBES genes in enterobacteria identified...
Inheritance of maize (Zea mays L.) leaf traits
December 2019
Maize leaf plays an important role in the synthesis of organic compounds. It hosts the interception of light energy and conversion to organic energy, and the capture and assimilation of carbon dioxide in order to produce food. In this study, we explore the heritability of the maize leaf length, leaf width, and the number of leaves of two maize genotypes, JNE and BLC. The estimates of heritability varied from 0.2 to...
Prevalence and genetic diversity of Plasmodium falciparum in patients attending regional health center in Daloa, Côte d'Ivoire
December 2019
Malaria is a parasitic disease defined as a major problem of public health in Côte d'Ivoire. One of the constraints of the control of this disease might be the genetic diversity of the main pathogen, Plasmodium falciparum. This study aims to evaluate the genetic diversity of Plasmodium falciparum in individuals at the regional health center in Daloa, Côte d’Ivoire. A total of 380 blood samples were...
Investigation of polymorphisms of exon 1 region of OsHKT1;5 gene in high yielding rice
June 2019
This study was carried out to analyze the polymorphic region of exon 1 of OsHKT1;5 of 22 high yielding rice varieties that are salt tolerant. The result of the phenotypic screening for salinity stress was done using the standard protocol of the International Rice Research Institute which recorded that all rice varieties developed very well under control condition, 85.38% were quite tolerant to salinity treatment of...
The characterization of Citrus sp. from Parang Island Karimunjawa based on morphological, DNA barcoding and nutritional analysis
September 2018
The Citrus sp. from Parang Island Karimunjawa is a wild type of Citrus plant that grow on salt area and exhibits a high level of vitamin C. Due to its ability, morphological, molecular and nutritional characterization needs to be carried out to improve its potential. The study was carried out at the Biotechnology Laboratory and the Integrated Laboratory of Diponegoro University from 2016 to 2017. The...
Use of expressed sequence tags-derived simple sequence repeat (SSR) markers for population studies of released and elite sweet potato
April 2018
Development of improved varieties of sweet potato to meet specific end users in the sub-Saharan African region has the potential of transforming the crop’s market, consumption and health benefits. Molecular biology techniques such as fingerprinting are key to crop improvement, and critical for numerous genetic studies. This study, carried out in Ghana, seeks to determine genetic relatedness among two populations...
Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization
March 2018
Plantains are important staples in sub-Saharan Africa, however, production has been saddled by several constraints including pests and diseases and lack of improved technologies. In regard to genetic study, morphological characterization has identified about 100 local accessions to exist in Ghana. However, limited molecular characterization has been done on plantains. Characterization based on morphologic...
De novo assembly and characterization of transcriptome and microsatellite marker development for Taro (Colocasia esculenta (L.) Schott.)
December 2017
Taro (Colocasia esculenta. (L.) Schott) is a genus of perennial plants that is widely distributed in the tropics or subtropics of Asia, Africa and America, which is the fourteenth most consumed vegetable of the world. However, molecular genetic research of Colocasia has been hindered by the insufficient genomic and transcriptome information. Here, the transcriptome of taro variety ‘Jingjiang Xiangsha’ from...
Broad sense heritability and genetic advance for grain yield and yield components of chickpea (Cicer arietinum L.) genotypes in western Ethiopia
November 2017
Genetic variability is a prerequisite to crop improvement. The objective of the present study was to assess and quantify the genetic variability, estimate heritability and genetic advance for yield and yield contributing characters of released and pipeline chickpea varieties based on agro- morphological traits. A total of 16 chickpea materials (8 improved varieties, 7 advanced lines, and one local check) were planted in...
Genetic polymorphism of vesicular monoamine transporter 1 gene (SLC18A1) in Emirati population
May 2017
Vesicular monoamine transporters (VMATs) are an important target for biological research in neuropsychiatric disorders. Recent studies indicated that VMAT1 is expressed in the brain, thus making transporter plausible candidate genes for neuropsychiatric disorders. Furthermore, several recent genetic case-control studies have documented an association between common missense variations in the VMAT1 gene and...
Biodiversity of uncultured bacteria in hypersaline lakes, Siwa oasis, Egypt, as determined by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes
April 2017
Bacterial populations within hypersaline lakes often exhibit uncultured unique species. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), followed by sequencing of 16S rRNA gene approach was applied in order to explore, for the first time, the bacterial communities within the hypersaline Lakes, Aghormy, Zeiton and Maraqi, Siwa Oasis, Egypt. The DGGE profile displayed 12 phylotypes of 16S rRNA...
The cichlid 16S gene as a phylogenetic marker: Limits of its resolution for analyzing global relationship
March 2017
The phylogenetic utility of the 16S gene in cichlids is assessed. Eighty-six (86) partial sequences belonging to 37 genera of cichlids from the Genbank was analyzed. The alignment had four hundred and sixty three (463) basepairs with 337 conserved sites and 126 variable sites. Base compositional bias is similar to that found in higher organism with Adenine having the highest average of 30.3%, followed by cytosine,...
Comparison of Lagostomus maximus amelogenin gene (AMELX) with other mammals
August 2016
The aim of this study was to compare the Lagostomus maximus AMEL sequence to assess their similarity with the AMEL genes from other mammals. Previously, a sequence of the AMEL gene of L. maximus which corresponds to the intron 3 of the human sequence for AMELX (AMEL on the X chromosome) was reported. The comparisons made, by the use of the algorithm provided by the NCBI (Basic Local Alignment Search Tool: BLAST) allowed...
Genetic diversity analysis in South African taro (Colocasia esculenta) accessions using molecular tools
August 2016
Taro [Colocasia esculenta (L) Schott] belongs to the family Araceae. It is an important staple food crop grown mainly by small scale farmers in many parts of the world. Taro is grown in South Africa from the coastal parts of the northern Eastern Cape to the KwaZulu-Natal north coast. Although it is an important staple in South Africa, very little information exists on the genetic diversity of the crop. Knowledge of the...
Recurrent mutation in the HMGCL gene in a family segregating HMG-CoA lyase deficiency
July 2016
The gene HMGCL encodes 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase. Mutations in HMG-CoA lyase cause HMG-CoA lyase deficiency (HMGCLD), which is an autosomal recessive congenital disorder of metabolism. This study was designed to detect mutation in the 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL) gene in a Saudi family segregating HMG-CoA lyase deficiency (HMGCLD).Methods: Clinical and molecular genetic analysis of a...
Genotypic restriction patterns of finger millet (Eleusine coracana (l.) Gaertn) landraces in northern Nigeria
May 2016
The present study was conducted to characterize the genetic diversity of 10 germplasm accessions of finger millet (Eleusine coracana (L) Gaertn) collected from diverse locations spread across the geographical zone of Northern Nigeria. The digest was carried out using the molecular marker Restriction Fragment Digest (RFD on agarose gel while EcoR1 and Hind III restriction enzymes were used to cut the genomic DNA at...
Association of the extracellular superoxide dismutase Ala40Thr polymorphism with type 2 diabetes mellitus and its complications in Lebanese population
January 2016
Free radical-induced damage plays a significant role in the development of type 2 diabetes and its complications. Extracellular superoxide dismutase (EC-SOD) or SOD3 is one of the important scavenger enzymes that protects against oxidative damage and the formation of free radicals. EC-SOD is a copper- and zinc-containing secretory enzyme located in tissues and primarily in the extracellular matrix. EC-SOD has been...
DYS459, DYS391, DYS388 and DYS19 genetic loci have high allelic frequency in patients with prostate cancer
August 2015
Prostate cancer is a significant disease in men, and a large number of individuals would benefit if risk factors that increase the susceptibility to develop prostate cancer could be established, which could aid in the early detection of the disease which is crucial for successful treatment. The first objective of this study was detection of allele frequencies of 12 Y-chromosome short tandem repeat loci from Iraqian...
Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin
July 2015
Alleviation of doxorubicin (DOX)-induced cytotoxicity and genotoxicity by leaf extract of bael also known as Aegle marmelos (AME) was studied in cultured Chinese hamster V79 cells. The optimum protective dose of AME was determined by treating V79 cells with different concentrations of AME before exposure to 10 µg/ml DOX and then by evaluating the cell survival and micronuclei frequency in the cytokinesis blocked...
Estimation of genetic parameters for first-lactation test-day milk yield in Holstein Friesian cows fitting random regression models
June 2015
In this study, the effects of varying order of Legendre polynomials (LP) for permanent environmental (Pe) variance structure on the estimates of genetic parameters for first-lactation milk yield were evaluated fitting random regression (RR) test-day animal models. The total data set included 6850 test-day milk yield records from 800 first-lactation Holstein Friesian cows that calved between 1997 and 2013 and the...
Isolation and sequence analysis of a putative MerR-type-transcriptional regulator and a multidrug efflux protein of Bacillus circulans ATCC 21588: As potential targets of therapeutics
May 2015
Mercury-type transcriptional regulators (MerR-transcriptional regulator) and major facilitator superfamily (MFS) transporters usually form an important sensor-response transport system in many microorganisms. This system has been shown to be involved in the regulation and transport (efflux) of a wide and diverse array of secondary metabolites including antimicrobial agents, dyes, chemicals, metals and evem harmful...
Effects of cytokinin types and their concentration on in vitro shoot induction and multiplication of korarima
April 2015
Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a herbaceous perennial plant that belongs to the family Zingiberaceae in which lack of a steady supply of quality planting material is one of the bottlenecks for the exploitation of the export potential. Thus, the use of micropropagation is suggested to alleviate these problems. In micropropagation, in vitro seed germination and multiplication of hypocotyls/shoot...
Evidence of over-dominance for sickle cell trait in a population sample from Buenaventura, Colombia
February 2015
Sickle cell disease is a hereditary disease caused by a hemoglobin variant, known as hemoglobin S. This hemoglobin variant has a high frequency in areas where malaria is prevalent, because heterozygous genotype has a protective action against malaria infection. We evaluated evidence of over-dominance or deviation from Hardy- Weinberg equilibrium for the HbS gene in Buenaventura, an afro-descendent population with actual...
Multivariate analysis of genetic divergence among Ethiopian mustard (Brassica Carinata A. Braun) landraces in Ethiopia
November 2014
Characterization of existing genetic variability is a prerequisite for further crop improvement activity. This study was designed to assess genetic variability among randomly selected Ethiopian mustard (Brassica Carinata A. Braun) genotypes from different agro ecology of Ethiopia. The present study was undertaken to determine nature of association of agronomic traits of 36 Ethiopian mustard (Brassica carinata) genotypes...
Genetic polymorphism of blood potassium in goat belonging to the different breeds in Mongolia
November 2014
In goats belonging to the different breeds and sub breed, the genetic polymorphism at the determinant locus of blood potassium was revealed by flame spectrophotometer method. The kalemic systems in those breeds were characterized by a polymorphism of middle level due to the existence of the two phenotypes and of three genotypes. The polymorphic character of this system is given by the distributional discontinuity of...
Use of simple sequence repeat (SSR) markers to establish genetic relationships among cassava cultivars released by different research groups in Ghanaian
October 2014
Cassava (Manihot esculenta) is an important staple crop widely cultivated in Ghana. The crop also has diverse industrial applications including starch, beer and alcohol productions. Knowledge about the state of the Ghanaian cassava genetic diversity and population structure is paramount in breeding programmes aimed at cultivar improvements or breeding of new cultivars for specific purposes. This study focused on the use...
Heliclobacter pylori associated chronic gastritis: Endoscopic and pathological findings, comparative study
March 2014
Helicobacter pylori have been established as a major etiologic factor in the pathogenesis of chronic gastritis, peptic ulcer disease and in the development of gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue lymphoma. This study was conducted on 100 patients. All underwent upper endoscopy, and antral and corpus, and duodenal biopsies were taken. Findings of endoscopic gastritis were observed in 83...
Challenges in conserving and utilizing plant genetic resources (PGR)
March 2014
The problems of food and income security are of global significance and are further compounded by precedential increase in world population resulting in overexploitation of natural resources and by extension plant genetic diversity. Plant genetic resources (PGR) refer to the heritable materials contained within and among plant species of present and potential value. In the recent past, genetic diversity found in...
Partitioning and distribution of random amplified polymorphic DNA (RAPD) variation among eggplant Solanum L. in Southwest Nigeria
January 2014
Solanum L., the largest genus of the Solanaceae family, vary morphologically, is diverse in number and is ecogeographically distributed. In Nigeria, previous studies had focused mainly on chromosome morphology, genome description and medicinal values, which are insufficient for genetic affinities. This study used four highly polymorphic random amplified polymorphic DNA primers to describe both the genetic relatedness...
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