The current study was conducted with the objectives of identifying Staphylococcus aureus from mastitis positive quarters and further screening of MRSA from positive cases for the bacteria. Identification of MRSA in dairy herd helps in determining its prevalence and provide information for further study. This study revealed insignificant (p≥0.05) prevalence of S. aureus in clinical (47.4%) and in subclinical (57.8%) mastitis. Amongst the isolates of Staphylococcus aureus, methicillin resistant Staphylococcus aureus was found in 38.9% of clinical and 28.1% of subclinical quarters of cows with insignificant (p>0.05) variation in the prevalence between the types of the mastitis. The current bacteriology based study revealed that Staphylococcus aureus was found prevalent in the study area. In vitro susceptibility test for the isolates against cefoxitin showed that where there is S. aureus, there is chance of finding methicillin resistant staphylococcus aureus

Keywords: prevalence, S. aureus, Meki, dairy farm, MRSA