International Journal of
Medicine and Medical Sciences

  • Abbreviation: Int. J. Med. Med. Sci.
  • Language: English
  • ISSN: 2006-9723
  • DOI: 10.5897/IJMMS
  • Start Year: 2009
  • Published Articles: 517

Full Length Research Paper

Role of histamine H2 receptor agonist and antagonist on liver function impairment in immunized rabbits

Trivendra Tripathi
  • Trivendra Tripathi
  • Department of Biochemistry, Faculty of Medicine, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh-202 002, UP, India.
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Mohammad Shahid
  • Mohammad Shahid
  • Department of Microbiology, Faculty of Medicine, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh-202 002, UP, India.
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Haris M. Khan
  • Haris M. Khan
  • Department of Microbiology, Faculty of Medicine, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh-202 002, UP, India.
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Rahat Ali Khan
  • Rahat Ali Khan
  • Department of Pharmacology, Faculty of Medicine, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh-202 002, UP, India.
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Mashiatullah Siddiqui
  • Mashiatullah Siddiqui
  • Department of Biochemistry, Faculty of Medicine, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh-202 002, UP, India.
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Abbas Ali Mahdi
  • Abbas Ali Mahdi
  • Department of Biochemistry, Chhatrapati Shahuji Maharaj Medical University, UP, Lucknow, India.
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Abida Malik
  • Abida Malik
  • Department of Microbiology, Faculty of Medicine, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh-202 002, UP, India.
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  • Article Number - 1996C7E374
  • Vol.2(12), pp. 395 - 401, December 2010
  •  Accepted: 07 October 2010
  •  Published: 31 December 2010

Abstract

This study was designed to explore the functional role of histamine H2-receptors agonist and antagonist in the development of hepatic function impairment in immunized rabbits. The study comprised six groups containing 18 rabbits each. Group III-VI received histamine (100 µg/kg, s.c.), H2R-agonist (amthamine, 10 µg/kg, s.c.), H2R-antagonist (ranitidine, 10 mg/kg, i.m.), and histamine (100 µg/kg, s.c.) plus H2R-antagonist (ranitidine, 10 mg/kg, i.m.), respectively, b.i.d. for 10 days. Group I (negative control) and group II (positive control) received sterile distilled water intramuscularly b.i.d. for 10 days. Groups II-VI were immunized on day 3 with intravenous injection of sheep red blood cells (1 × 10cells/ml). Blood samples were collected prior to immunization day 0, as well as on days 7, 14, 21, 28, and 58 post-immunization. Biochemical parameters ALT, AST, ALP, and bilirubin were determined. On each experimental day, the mean values of serum enzymes (AST, ALT, and ALP) and bilirubin (TB, DB, and IB) in negative and positive controls showed no significant changes while in group III (histamine), IV (amthamine), V (ranitidine), and VI (ranitidine+histamine), these enzymes and bilirubin levels showed significant changes (p < 0.05), when compared with their values within group. The levels of serum enzymes and bilirubin showed significant difference (p < 0.05) in the group of histamine, amthamine, ranitidine, and ranitidine+histamine on each experimental day, when compared with the corresponding values of each other, and also compared with the corresponding values of negative and positive controls. Histamine, amthamine, ranitidine, and combination of ranitidine+histamine cause liver function impairment in terms of altered serum enzymes and bilirubin levels.

Key words: Histamine, HTMT, Agonist, Antagonist, ALT, AST, ALP, bilirubin, rabbit.