International Journal of
Plant Physiology and Biochemistry

  • Abbreviation: Int. J. Plant Physiol. Biochem.
  • Language: English
  • ISSN: 2141-2162
  • DOI: 10.5897/IJPPB
  • Start Year: 2009
  • Published Articles: 113

Full Length Research Paper

Study of inter-specific relationship in six species of Sesbania Scop. (Leguminosae) through RAPD and ISSR markers

Manoj Kumar Bisoyi1, Laxmikanta Acharya2, Arup Kumar Mukherjee3,4* and Chandra Panda Pratap2
  1Department of Botany and Biotechnology, Khallikote Autonomous College, Berhampur, Orissa, India. 2Division of Taxonomy and Conservation, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Orissa, India. 3DNA Fingerprinting Laboratory, Division of Plant Biotechnology, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Orissa, India. 4Central Institute for Cotton Research, Shankar Nagar PO, Nagpur-440010, Maharashtra, India.    
Email: [email protected]

  •  Accepted: 08 March 2010
  •  Published: 31 May 2010



Six species of Sesbania were fingerprinted using RAPD and ISSR markers. Both the markers yielded a total of 249 bands out of which 243 were polymorphic in nature indicating high degrees of genetic diversity in the genus. Cluster analysis using the combined data revealed segregation of the lone species Sesbania grandiflora from the rest of the species. High boot strap values in the dendrograms show the accuracy and authenticity of the result. All other five species got separated to a distinct cluster. This supports the taxonomic division of the genus Sesbania into Agati Desv. comprising the only species S. grandiflora (Linn.) Poir. and sub-genus Eusesbania Baker containing all other species.


Key words: Sesbania, RAPD, ISSR, phenogram.