International Journal of
Plant Physiology and Biochemistry

  • Abbreviation: Int. J. Plant Physiol. Biochem.
  • Language: English
  • ISSN: 2141-2162
  • DOI: 10.5897/IJPPB
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Influence of media and growth regulators on regeneration and morphological characteristics of strawberry cvs Kurdistan and Merck (Fragaria x ananassa Duch.)

Ali-Akbar Mozafari1* and Mohammad Gerdakaneh2
1Department of Horticultural Science, Faculty of Agriculture, University of Kurdistan, Iran. 2Agricultural and Natural Recourses Research Center of Kermanshah Province, Iran.
Email: [email protected]

  •  Accepted: 20 March 2012
  •  Published: 30 April 2012

Abstract

To evaluate the best media and growth regulator combinations for the regeneration and morphological characteristics of strawberry, meristems of Kurdistan and Merck strawberry cultivars were cultured on Murashige and Skoog (1962),  Gamborg et al. (1968) and Nitsch and Nitsch (1969) media supplemented with various growth regulator combinations including B1: (BA 1.0 mg/l + IBA 0.05 mg/l + GA3 0.05 mg/l), B2: (Kinetin 5 mg/l + 2,4-D 0.5 mg/l + GA3 0.05 mg/l) and B3: (BA 2 mg/l + IBA 0.2 mg/l + GA3  0.05 mg/l). The largest number of shoots was obtained on NN medium supplemented with B1 followed by MS and B5 media with the same growth regulator combination. In the media, B2 increased shoot elongation rate in both cultivars, but caused a low frequency of shoot formation. There was significant difference among various media and growth regulator combinations in regards to morphological traits such as leaf length, width of terminal leaflets and number of terminal leaflets teeth, whereas little differences were observed in the length of terminal leaflets and the length of terminal leaflet petiolate.

 

Key words: Strawberry, growth regulator, regeneration, cultivar.

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