Journal of
Agricultural Biotechnology and Sustainable Development

  • Abbreviation: J. Agric. Biotech. Sustain. Dev.
  • Language: English
  • ISSN: 2141-2340
  • DOI: 10.5897/JABSD
  • Start Year: 2009
  • Published Articles: 139

Full Length Research Paper

Biofertilizer: A novel approach for agriculture

Swapnil D. Topre4*, Sreejit S. Panikar1, Sarita U. Mahajani2 and Satish B. Patil3
  1Department of Biotechnology, Rajmata Jijau Shikshan Prasarak Mandal’s (RJSPM’s) College, Bhosari, Pune 411039, Maharashtra, India. 2Lokmangal Biotechnology College, Wadala, North Solapur 413 222, India. 3Department of Environmental Science, University of Pune, Ganeshkhind, Pune 411007, Maharashtra, India. 430/4, Golf Residency, Near IBM, Yerwada, Pune-411006, Maharashtra, India.
Email: [email protected]

  •  Accepted: 18 January 2011
  •  Published: 31 December 2011



The nodulation activity in some legume plants was estimated using two nitrogen fixing microorganisms that produced and introduced in different growth conditions. Two isolates of agriculturally important nitrogen fixing microorganisms, Rhizobium meliloti and Azospirillium lipoferum were isolated from Western Maharashtra soil habitat. The standard isolation technique was carried out in laboratory and was characterized by 16S rDNA method. The growth of mentioned microorganisms was studied at different pH, the optimal pH was found to be 6.5 and 7 for R. meliloti and A. lipoferum respectivelyThe nodulation activity of R. meliloti was confirmedCarrier plays an important role in maintaining sufficient shelf life, so,survival of mentioned microorganisms in different carriers at 28°C was deduced. However, lignite was found to be the most efficient carrier for R. meliloti and A. lipoferum to introduce as biofertilizer. The mentioned biofertilizer was checked for colony count to meet the standard requirement (that is, 107cells/g). Furthermore, the mentioned microorganisms were multiplied for mass production using lignite as carrier.


Key words:  Rhizobium melilotiAzospirillium lipoferum, biofertilizer, lignite.