Journal of
Agricultural Biotechnology and Sustainable Development

  • Abbreviation: J. Agric. Biotech. Sustain. Dev.
  • Language: English
  • ISSN: 2141-2340
  • DOI: 10.5897/JABSD
  • Start Year: 2009
  • Published Articles: 143

Full Length Research Paper

In vitro regeneration of Acacia nilotica (L.) Willd. ex Del from nodes on B5 medium

Gabriel Samake1,2*, Fousseni Folega3,4, Hamidou Senou2 and Hua-Fang Wang1
  1College of Biological Sciences and Biotechnology, Beijing Forestry University. Beijing 226 100083. P. R. China. 2College of Agronomy, IPR / IFRA, Katibougou Bp: 06, Koulikoro, Mali. 3Key Laboratory for Sylviculture and Conservation of Ministry of Education Beijing Forestry University Beijing – China. 4Laboratoire d’Ecologie végétale et de Botanique, Faculté des Sciences, Université de Lomé, BP: 1515 Lomé-Togo.
Email: [email protected]

  •  Accepted: 13 May 2011
  •  Published: 30 June 2011



Acacia nilotica (L.) Willd. ex Del epicotyls buds were initiated in a medium supplemented by a combination of naphthalene acetic acid NAA (0.1 mg/L), benzyl amino purine BA (1.5 mg/L) and gibberellic acid GA (1.5 mg/L) were then used to produce a factorial experiment on B5 medium supplemented with auxin indole-3-butyric acid (IBA) and cytokinin BA with five levels (0.0, 0.1, 0.5, 1.0 and 1.5 mg/L) of each. At 0.5 mg/L level BA produced the highest number of nodes (5.8) and longest stem length (55.83 mm). However, the interaction between the two hormones (IBA and BA) had a negative effect on the growth and rooting of the shoots. High level, on the other hand, had a shortening effect on the internodes.  BA used only with high doses has an effect of shortening on plants. Level 1.0 mg/L of IBA produced 100% rooted plants with short roots. Shoots are thin and have general growth retardation.


Key words: Acacia nilotica, regeneration, microcutting, rooting, node.