Acacia nilotica (L.) Willd. ex Del epicotyls buds were initiated in a medium supplemented by a combination of naphthalene acetic acid NAA (0.1 mg/L), benzyl amino purine BA (1.5 mg/L) and gibberellic acid GA (1.5 mg/L) were then used to produce a factorial experiment on B5 medium supplemented with auxin indole-3-butyric acid (IBA) and cytokinin BA with five levels (0.0, 0.1, 0.5, 1.0 and 1.5 mg/L) of each. At 0.5 mg/L level BA produced the highest number of nodes (5.8) and longest stem length (55.83 mm). However, the interaction between the two hormones (IBA and BA) had a negative effect on the growth and rooting of the shoots. High level, on the other hand, had a shortening effect on the internodes. BA used only with high doses has an effect of shortening on plants. Level 1.0 mg/L of IBA produced 100% rooted plants with short roots. Shoots are thin and have general growth retardation.
Key words: Acacia nilotica, regeneration, microcutting, rooting, node.
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