Journal of
Agricultural Biotechnology and Sustainable Development

  • Abbreviation: J. Agric. Biotech. Sustain. Dev.
  • Language: English
  • ISSN: 2141-2340
  • DOI: 10.5897/JABSD
  • Start Year: 2009
  • Published Articles: 134

Table of Content: May 2014; 6(2)

May 2014

Agrobacterium mediated transfer of nptII and gus genes in Camellia assamica

The present work is an attempt to develop transgenic hairy root system in Camellia assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and neomycin phosphotransferase (nptII) as selection marker. The transformed hairy roots were grown in phytohormone free MS media in the presence of kanamycin 50...

Author(s): Neelakshi Bhattacharyya, Hijam Ranjit Singh, Niraj Agarwala, Prasenjit Bhagawati, Giasuddin Ahmed and Sudripta Das